FIGURE 3

The social preference test reveals recovery of vision within 7 days after light lesion. (A) Experimental timeline. Recordings were performed in light on conditions in presence of a social stimulus before light lesion (control) and at 1, 3, 7, 14, and 28 days post lesion (dpl). (B) Heat maps of one representative individual fish show that the time spent in the social zone decreases significantly following light lesion and returned to baseline from 7 dpl onwards. (C) Quantification of the time spent in the social zone confirms a significant decrease at 1 and 3 dpl (4.6 ± 2% and 10.8 ± 8.8%) and the return to control levels (34.5 ± 8.4%) after 7 dpl (7 dpl: 23.8 ± 14%, 14 dpl: 34.8 ± 8.7%, 28 dpl: 34.1 ± 4.7%). Quantifications of the distance moved (D), velocity (E) and frequency in zone (F) did not reveal any significant changes within the observed timeframe. Statistics: all data are represented as mean ± SD, one-way ANOVA with Dunnett’s multiple comparison test against control, p < 0.05 (*); 0.01 (**); 0.001 (***) or 0.0001 (****).