FIGURE

Fig. 7

ID

ZDB-FIG-210331-7

Publication

Zeng et al., 2021 - Injury-induced Cavl-expressing cells at lesion rostral side play major roles in spinal cord regeneration

Other Figures

All Figure Page

Back to All Figure Page

Fig. 7

Overexpression of cav1 could drive caudal-SrRCs to extend longer axonal neurites in vivo. Rostral- and caudal-SrRCs were separately collected from SCI-embryos, and primary culture (PC) was carried out for 48 h. Afterwards, plasmid containing cav1 cDNA was transfected into caudal-SrRCs. Neurites developed from cultured SrRCs after plasmid was transfected for 24 h (T24) and 48 h (T48) were morphologically examined: (a) non-transfected rostral-SrRCs, (b) non-transfected caudal-SrRCs, (c) caudal-SrRCs transfected with pCS2-vector and (d) caudal-SrRCs transfected with pCS2 containing cav1 and cav1-flag cDNA. Right panel of each figure shows the amplified image of the box area indicated on left panel. Arrowheads indicate the extended axons. (e) The average neurite length developed from SrRCs cultured for 48 h after transfection was calculated from 200 cultured SrRCs. Statistical analysis was based on t-test at **p < 0.01 and ***p < 0.001 significance.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Open Biol.