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Fig. 7 Overexpression of cav1 could drive caudal-SrRCs to extend longer axonal neurites in vivo. Rostral- and caudal-SrRCs were separately collected from SCI-embryos, and primary culture (PC) was carried out for 48 h. Afterwards, plasmid containing cav1 cDNA was transfected into caudal-SrRCs. Neurites developed from cultured SrRCs after plasmid was transfected for 24 h (T24) and 48 h (T48) were morphologically examined: (a) non-transfected rostral-SrRCs, (b) non-transfected caudal-SrRCs, (c) caudal-SrRCs transfected with pCS2-vector and (d) caudal-SrRCs transfected with pCS2 containing cav1 and cav1-flag cDNA. Right panel of each figure shows the amplified image of the box area indicated on left panel. Arrowheads indicate the extended axons. (e) The average neurite length developed from SrRCs cultured for 48 h after transfection was calculated from 200 cultured SrRCs. Statistical analysis was based on t-test at **p < 0.01 and ***p < 0.001 significance.