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ZDB-FIG-210216-10

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Das et al., 2020 - Cortisol rapidly stimulates calcium waves in the developing trunk muscle of zebrafish

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Fig. 2

Fig. 2. Cortisol rapidly stimulates [(Ca²⁺)i] wave in zebrafish muscle. (a) Representative images of the developing trunk muscle (DTM) loaded with the ratiometric dye FURA-2AM either with (100 ng/ml) or without the addition of cortisol. (b) A representative line graph of the temporal calcium dynamics in response to cortisol (CORT) addition over a 5 min period. FURA-2AM intensity was recorded at 5 s intervals. (c) Graph showing the ratiometric FURA-2AM intensity with different concentrations of cortisol (10, 50 and 100 ng/ml) at 30 s post-cortisol addition. All bars represent mean ± SEM (n = 4–5 independent embryos); bars with different letters are significantly different; one-way ANOVA; Holm Sidak post hoc test; p < 0.05.

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Reprinted from Molecular and Cellular Endocrinology, 520, Das, C., Faught, E., Vijayan, M.M., Cortisol rapidly stimulates calcium waves in the developing trunk muscle of zebrafish, 111067, Copyright (2020) with permission from Elsevier. Full text @ Mol. Cell. Endocrinol.