PUBLICATION
Cortisol rapidly stimulates calcium waves in the developing trunk muscle of zebrafish
- Authors
- Das, C., Faught, E., Vijayan, M.M.
- ID
- ZDB-PUB-201120-3
- Date
- 2020
- Source
- Molecular and Cellular Endocrinology 520: 111067 (Review)
- Registered Authors
- Faught, Erin, Vijayan, Mathilakath
- Keywords
- CRAC channel, Glucocorticoid receptor, Glucocorticoids, Nongenomic steroid signaling, Orai1, Stress
- MeSH Terms
-
- Animals
- Calcium/metabolism
- Calcium Channels/metabolism
- Calcium Signaling/drug effects*
- Cell Membrane/drug effects
- Cell Membrane/metabolism
- Embryo, Nonmammalian/drug effects
- Embryo, Nonmammalian/metabolism
- Hydrocortisone/pharmacology*
- Imaging, Three-Dimensional
- Ion Channel Gating/drug effects
- Muscles/drug effects
- Muscles/physiology*
- ORAI1 Protein/metabolism
- Receptors, Glucocorticoid/metabolism
- Stress, Physiological/drug effects
- Torso/physiology*
- Zebrafish/embryology*
- Zebrafish Proteins/metabolism
- PubMed
- 33129866 Full text @ Mol. Cell. Endocrinol.
Citation
Das, C., Faught, E., Vijayan, M.M. (2020) Cortisol rapidly stimulates calcium waves in the developing trunk muscle of zebrafish. Molecular and Cellular Endocrinology. 520:111067.
Abstract
Glucocorticoids (GCs) play a role in stress coping by activating the glucocorticoid receptor (GR), a ligand-bound transcription factor. GCs also exert rapid effects that are nongenomic by modulating second messenger signaling, including Ca2+. However, the mechanism of action of GCs in modulating cytoplasmic free calcium level ([Ca2+]i) is unclear. We hypothesized that cortisol increases ([Ca2+]i) in zebrafish (Danio rerio) muscle, and this is independent of GR activation. Indeed, cortisol rapidly stimulated ([Ca2+]i) rise in the developing trunk muscle (DTM), and this response was not abolished in the GR knockout zebrafish. The rapid cortisol-induced ([Ca2+]i) rise was reduced with EGTA, and completely abolished by the pharmacological inhibition of the calcium release-activated calcium channel (CRACC). Also, cortisol stimulation rapidly increased the expression of Orai1, the pore forming protein subunit of CRACC, in the DTM. Altogether, rapid nongenomic action of cortisol on muscle function may involve Ca2+ signaling by CRACC gating in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping