FIGURE

Fig. 3

ID

ZDB-FIG-200813-5

Publication

Lex et al., 2020 - Telomere shortening produces an inflammatory environment that increases tumor incidence in zebrafish

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Fig. 3

G2 tert−/− larvae with very short telomeres exhibit increased melanoma expansion. (A) Experimental design for melanoma allotransplants in zebrafish larvae. Melanoma tumors were dissected from Tg(mitfa:HRASG12V; β-actin:GFP) zebrafish and cells dissociated. HRAS melanoma cells were then injected into blood circulation of 2-dpf zebrafish larvae. Larvae were kept for 7-d-postinjection (7-dpi). (B) Representative images of HRAS melanoma cells spread (green) in WT or G2 tert−/− larvae at 7 dpi. (Scale bars: 0.5 mm.) (C) Time course of HRAS melanoma cells spread in a group of WT and G2 tert−/− larvae (P < 0.01 at 7 dpi; WT n = 10; G2 tert−/− n = 11). (D) Melanoma tumors are more disseminated in G2 tert−/− larvae (*P < 0.05; WT n = 32; G2 tert−/− n = 31). A linear regression of three time points (1, 4, and 7 dpi) was used to calculate the slope of melanoma expansion over time. Each dot represents one larvae allotransplant.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA