ZFIN ID: ZDB-FIG-200718-60
Wu et al., 2020 - Low Molecular Weight Fucoidan Prevents Radiation-Induced Fibrosis and Secondary Tumors in a Zebrafish Model. Cancers   12(6) Full text @ Cancers
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Conditions:
Anatomical Term:
Stage: Adult
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage: Adult

Figure 3

Expression levels of lipogenic factor and lipogenesis enzyme genes in adult zebrafish treated with Oligo-Fucoidan and radiation. (A) The expression of lipogenic factors (pparg, srebp1, and mlxlp) in HBx,src transgenic fish with diet-induced obesity plus 40 Gy irradiation (DIO + 40 Gy) or treatment of Oligo-Fucoidan before 40 Gy irradiation (DIO + OF + 40 Gy). (B) The expression of lipogenesis enzyme 1-acylglycerol-3-phosphate O-acyltransferase 4 (lysophosphatidic acid acyltransferase, delta) (agpat4, phospholipid phosphatase 1a (plpp1a)), transcript variant X1, mRNA (pap), and fatty acid synthase (fasn) in HBx,src transgenic fish with diet-induced obesity plus 40 Gy irradiation (DIO + 40 Gy) or treatment of Oligo-Fucoidan before 40 Gy irradiation (DIO + OF + 40 Gy). (C). The expression of collagen, type I, alpha 1a (col1a1), connective tissue growth factor a (ctgfa), and heparanase (hpse) in HBx,src transgenic fish with diet-induced obesity plus 40 Gy irradiation (DIO + 40Gy) or treatment of Oligo-Fucoidan before 40 Gy irradiation (DIO + OF + 40 Gy). (D). The expression of cyclin E1 (ccne1), cyclin-dependent kinase 1 (cdk1), and cyclin-dependent kinase 2 (cdk2) in HBx, src transgenic fish with diet-induced obesity plus 40 Gy irradiation (DIO + 40 Gy) or treatment of Oligo-Fucoidan before 40 Gy irradiation (DIO + OF + 40 Gy). (E). The expression of col1a1, ctgfa, and hpse in HBx, src, p53-transgenic fish with diet-induced obesity plus 10 Gy irradiation (DIO + 10 Gy) or treatment of Oligo-Fucoidan before 10 Gy irradiation (DIO + OF + 10 Gy). (F). The expression of ccne1, cdk1, and cdk2 in HBx,src,p53-transgenic fish with diet-induced obesity plus 10 Gy irradiation (DIO + 10 Gy) or treatment of Oligo-Fucoidan before 10 Gy irradiation (DIO + OF + 10 Gy). The data are presented as dot plots with a horizontal line for the mean and are repeated in triplicate. The statistical significance was calculated using Student’s t-test (* p < 0.05, ** p < 0.01, ns: non-significant).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
agpat4 nn1002Tg; nn1003Tg (AB) increased food availability, particle radiation Adult liver RTPCR
nn1002Tg; nn1003Tg (AB) chemical treatment by gavage: fucoidan, increased food availability, particle radiation Adult liver RTPCR
ccne1 nn1002Tg; nn1003Tg (AB) increased food availability, particle radiation Adult liver RTPCR
nn1002Tg; nn1003Tg (AB) chemical treatment by gavage: fucoidan, increased food availability, particle radiation Adult liver RTPCR
tp53zdf1/zdf1; nn1002Tg; nn1003Tg (AB) increased food availability, particle radiation Adult liver RTPCR
tp53zdf1/zdf1; nn1002Tg; nn1003Tg (AB) chemical treatment by gavage: fucoidan, increased food availability, particle radiation Adult liver RTPCR
cdk1 nn1002Tg; nn1003Tg (AB) increased food availability, particle radiation Adult liver RTPCR
nn1002Tg; nn1003Tg (AB) chemical treatment by gavage: fucoidan, increased food availability, particle radiation Adult liver RTPCR
tp53zdf1/zdf1; nn1002Tg; nn1003Tg (AB) increased food availability, particle radiation Adult liver RTPCR
tp53zdf1/zdf1; nn1002Tg; nn1003Tg (AB) chemical treatment by gavage: fucoidan, increased food availability, particle radiation Adult liver RTPCR
cdk2 nn1002Tg; nn1003Tg (AB) increased food availability, particle radiation Adult liver RTPCR
nn1002Tg; nn1003Tg (AB) chemical treatment by gavage: fucoidan, increased food availability, particle radiation Adult liver RTPCR
tp53zdf1/zdf1; nn1002Tg; nn1003Tg (AB) increased food availability, particle radiation Adult liver RTPCR
tp53zdf1/zdf1; nn1002Tg; nn1003Tg (AB) chemical treatment by gavage: fucoidan, increased food availability, particle radiation Adult liver RTPCR
col1a1a nn1002Tg; nn1003Tg (AB) increased food availability, particle radiation Adult liver RTPCR
nn1002Tg; nn1003Tg (AB) chemical treatment by gavage: fucoidan, increased food availability, particle radiation Adult liver RTPCR
tp53zdf1/zdf1; nn1002Tg; nn1003Tg (AB) increased food availability, particle radiation Adult liver RTPCR
tp53zdf1/zdf1; nn1002Tg; nn1003Tg (AB) chemical treatment by gavage: fucoidan, increased food availability, particle radiation Adult liver RTPCR
ccn2a nn1002Tg; nn1003Tg (AB) increased food availability, particle radiation Adult liver RTPCR
nn1002Tg; nn1003Tg (AB) chemical treatment by gavage: fucoidan, increased food availability, particle radiation Adult liver RTPCR
tp53zdf1/zdf1; nn1002Tg; nn1003Tg (AB) increased food availability, particle radiation Adult liver RTPCR
tp53zdf1/zdf1; nn1002Tg; nn1003Tg (AB) chemical treatment by gavage: fucoidan, increased food availability, particle radiation Adult liver RTPCR
fasn nn1002Tg; nn1003Tg (AB) increased food availability, particle radiation Adult liver RTPCR
nn1002Tg; nn1003Tg (AB) chemical treatment by gavage: fucoidan, increased food availability, particle radiation Adult liver RTPCR
hpse nn1002Tg; nn1003Tg (AB) increased food availability, particle radiation Adult liver RTPCR
nn1002Tg; nn1003Tg (AB) chemical treatment by gavage: fucoidan, increased food availability, particle radiation Adult liver RTPCR
tp53zdf1/zdf1; nn1002Tg; nn1003Tg (AB) increased food availability, particle radiation Adult liver RTPCR
tp53zdf1/zdf1; nn1002Tg; nn1003Tg (AB) chemical treatment by gavage: fucoidan, increased food availability, particle radiation Adult liver RTPCR
mlxip nn1002Tg; nn1003Tg (AB) increased food availability, particle radiation Adult liver RTPCR
nn1002Tg; nn1003Tg (AB) chemical treatment by gavage: fucoidan, increased food availability, particle radiation Adult liver RTPCR
pap nn1002Tg; nn1003Tg (AB) increased food availability, particle radiation Adult liver RTPCR
nn1002Tg; nn1003Tg (AB) chemical treatment by gavage: fucoidan, increased food availability, particle radiation Adult liver RTPCR
pparg nn1002Tg; nn1003Tg (AB) increased food availability, particle radiation Adult liver RTPCR
nn1002Tg; nn1003Tg (AB) chemical treatment by gavage: fucoidan, increased food availability, particle radiation Adult liver RTPCR
srebf1 nn1002Tg; nn1003Tg (AB) increased food availability, particle radiation Adult liver RTPCR
nn1002Tg; nn1003Tg (AB) chemical treatment by gavage: fucoidan, increased food availability, particle radiation Adult liver RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
nn1002Tg; nn1003Tg (AB) increased food availability, particle radiation Adult liver ccn2a expression increased amount, abnormal
Adult liver ccne1 expression increased amount, abnormal
Adult liver cdk1 expression increased amount, abnormal
Adult liver cdk2 expression increased amount, abnormal
Adult liver col1a1a expression increased amount, abnormal
Adult liver hpse expression increased amount, abnormal
Adult liver mlxip expression increased amount, abnormal
Adult liver pap expression increased amount, abnormal
Adult liver pparg expression increased amount, abnormal
Adult liver srebf1 expression increased amount, abnormal
nn1002Tg; nn1003Tg (AB) chemical treatment by gavage: fucoidan, increased food availability, particle radiation Adult liver ccn2a expression amount, ameliorated
Adult liver ccne1 expression amount, ameliorated
Adult liver cdk1 expression amount, ameliorated
Adult liver cdk2 expression amount, ameliorated
Adult liver col1a1a expression amount, ameliorated
Adult liver hpse expression amount, ameliorated
Adult liver mlxip expression amount, ameliorated
Adult liver pap expression amount, ameliorated
Adult liver pparg expression amount, ameliorated
Adult liver srebf1 expression amount, ameliorated
tp53zdf1/zdf1; nn1002Tg; nn1003Tg (AB) increased food availability, particle radiation Adult liver ccn2a expression increased amount, abnormal
Adult liver ccne1 expression increased amount, abnormal
Adult liver cdk1 expression increased amount, abnormal
Adult liver cdk2 expression increased amount, abnormal
Adult liver col1a1a expression increased amount, abnormal
Adult liver hpse expression increased amount, abnormal
tp53zdf1/zdf1; nn1002Tg; nn1003Tg (AB) chemical treatment by gavage: fucoidan, increased food availability, particle radiation Adult liver ccn2a expression amount, ameliorated
Adult liver ccne1 expression amount, ameliorated
Adult liver cdk1 expression amount, ameliorated
Adult liver cdk2 expression amount, ameliorated
Adult liver col1a1a expression amount, ameliorated
Adult liver hpse expression amount, ameliorated
Acknowledgments:
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