ZFIN ID: ZDB-FIG-200718-58
Wu et al., 2020 - Low Molecular Weight Fucoidan Prevents Radiation-Induced Fibrosis and Secondary Tumors in a Zebrafish Model. Cancers   12(6) Full text @ Cancers
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Conditions:
Anatomical Term:
Stage: Adult
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage: Adult

Figure 1

Expression levels of apoptosis, fibrosis, and cell proliferation-related genes in adult wild-type zebrafish treated with Oligo-Fucoidan and radiation. (A) The expression of B-cell lymphoma 2 (bcl2)-associated agonist of cell death (bad), bcl2-associated X protein a (baxa), apoptosis-related cysteine peptidase 3a (caspase 3a), apoptosis-related cysteine peptidase 8 (caspase 8), apoptosis antigen 1 (fas), c-Jun N-terminal kinase 1 (jnk-1), mitogen-activated protein kinase 8a (p38(MAPK)) in wild-type fish treated with 40 Gy irradiation (40 Gy) or treatment of Oligo-Fucoidan before 40 Gy irradiation (OF + 40Gy). (B) The expression of collagen, type I, alpha 1a (col1a1), connective tissue growth factor a (ctgfa), heparanase (hpse) in wild-type fish treated with 40 Gy irradiation (40 Gy) or treatment of Oligo-Fucoidan before 40 Gy irradiation (OF + 40 Gy). (C) The expression of cyclin E1 (ccne1), cyclin-dependent kinase 1 (cdk1), and cyclin-dependent kinase 2 (cdk2) in wild-type fish treated with 40 Gy irradiation (40 Gy) or treatment of Oligo-Fucoidan before 40 Gy irradiation (OF + 40 Gy). (D) The expression of col1a1, ctgfa, and hpse in wild-type fish treated with 10 Gy irradiation (10 Gy) or treatment of Oligo-Fucoidan before 10 Gy irradiation (OF + 10 Gy). The data are presented as dot plots with a horizontal line for the mean and are repeated in triplicate. The statistical significance was calculated using Student’s t-test (* p < 0.05, ** p < 0.01, ns: non-significant).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
badb WT chemical treatment by gavage: fucoidan, particle radiation Adult liver RTPCR
WT particle radiation Adult liver RTPCR
baxa WT chemical treatment by gavage: fucoidan, particle radiation Adult liver RTPCR
WT particle radiation Adult liver RTPCR
casp3a WT chemical treatment by gavage: fucoidan, particle radiation Adult liver RTPCR
WT particle radiation Adult liver RTPCR
casp8 WT chemical treatment by gavage: fucoidan, particle radiation Adult liver RTPCR
WT particle radiation Adult liver RTPCR
ccne1 WT chemical treatment by gavage: fucoidan, particle radiation Adult liver RTPCR
WT particle radiation Adult liver RTPCR
cdk1 WT chemical treatment by gavage: fucoidan, particle radiation Adult liver RTPCR
WT particle radiation Adult liver RTPCR
cdk2 WT chemical treatment by gavage: fucoidan, particle radiation Adult liver RTPCR
WT particle radiation Adult liver RTPCR
col1a1a WT chemical treatment by gavage: fucoidan, particle radiation Adult liver RTPCR
WT particle radiation Adult liver RTPCR
ccn2a WT chemical treatment by gavage: fucoidan, particle radiation Adult liver RTPCR
WT particle radiation Adult liver RTPCR
fas WT chemical treatment by gavage: fucoidan, particle radiation Adult liver RTPCR
WT particle radiation Adult liver RTPCR
hpse WT chemical treatment by gavage: fucoidan, particle radiation Adult liver RTPCR
WT particle radiation Adult liver RTPCR
mapk8a WT chemical treatment by gavage: fucoidan, particle radiation Adult liver RTPCR
WT particle radiation Adult liver RTPCR
mapk14a WT chemical treatment by gavage: fucoidan, particle radiation Adult liver RTPCR
WT particle radiation Adult liver RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment by gavage: fucoidan, particle radiation Adult liver badb expression amount, ameliorated
Adult liver baxa expression amount, ameliorated
Adult liver casp3a expression amount, ameliorated
Adult liver casp8 expression amount, ameliorated
Adult liver ccn2a expression amount, ameliorated
Adult liver ccne1 expression increased amount, abnormal
Adult liver cdk1 expression amount, ameliorated
Adult liver cdk2 expression amount, ameliorated
Adult liver col1a1a expression amount, ameliorated
Adult liver fas expression amount, ameliorated
Adult liver hpse expression amount, ameliorated
Adult liver mapk14a expression amount, ameliorated
Adult liver mapk8a expression amount, ameliorated
WT particle radiation Adult liver badb expression increased amount, abnormal
Adult liver baxa expression increased amount, abnormal
Adult liver casp3a expression increased amount, abnormal
Adult liver casp8 expression increased amount, abnormal
Adult liver ccn2a expression increased amount, abnormal
Adult liver ccne1 expression increased amount, abnormal
Adult liver cdk1 expression increased amount, abnormal
Adult liver cdk2 expression increased amount, abnormal
Adult liver col1a1a expression increased amount, abnormal
Adult liver fas expression increased amount, abnormal
Adult liver hpse expression increased amount, abnormal
Adult liver mapk14a expression increased amount, abnormal
Adult liver mapk8a expression increased amount, abnormal
Acknowledgments:
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