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Fig. 5

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ZDB-FIG-200604-9

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Green et al., 2020 - Wnt signaling regulates neural plate patterning in distinct temporal phases with dynamic transcriptional outputs

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Fig. 5

The neural plate responds dynamically to global Wnt activation during gastrulation. In situ hybridizations to otx2b (A–H), otx1 (I–P), gbx1 (Q–X), and her5 (Y–F’) on bud stage hs:wnt8a/+ and +/+ sibling (control) embryos. Dorsal views, animal pole up. Time of heat shock initiation is indicated above respective columns. Note repression of otx2 (B) and otx1 (J) after 4.7 hpf heat shock, concomitant with anterior expansion of gbx1 (R) and disorganized her5+ cells (Z). otx2b repression occurs after 6 hpf heat shock (D), though otx1 does not (L), gbx1 is mildly increased in the posterior neural plate (T), and her5 shows scattered expression anterior to normal domain (B’). Expression of all markers is relatively normal after 8 hpf heat shocks (F,N,V) with slight anterior expansion of her5 into prospective telencephalic domain (D’). otx2b, otx1 and gbx1 are normal after 9 hpf heat shock (H,P,X), while her5 is reduced (F’).

Expression Data

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Antibody Labeling

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Acknowledgments

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Reprinted from Developmental Biology, 462(2), Green, D.G., Whitener, A.E., Mohanty, S., Mistretta, B., Gunaratne, P., Yeh, A.T., Lekven, A.C., Wnt signaling regulates neural plate patterning in distinct temporal phases with dynamic transcriptional outputs, 152-164, Copyright (2020) with permission from Elsevier. Full text @ Dev. Biol.