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- ZDB-FIG-200604-1
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- Green et al., 2020 - Wnt signaling regulates neural plate patterning in distinct temporal phases with dynamic transcriptional outputs
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Temporal analysis of Wnt signaling modulation by the hs:dkk1b and hs:wnt8a transgenes. In situ hybridizations to dkk1b (A-E) or axin2 (F-J) in hs:dkk1b. Time of fixation after heat shock (hours post heat shock, hph) is indicated in the lower right. White lines separate wild type embryos (left of line) from transgenic embryos (right of line). dkk1b staining is strong up to 2 hph (A-C), declines by 3.5 hph (D), and is similar to wild type by 4 hph (E). axin2 is visibly reduced up to 3.5 hph (F-H), and recovers to normal levels between 4 hph and 5 hph (I,J). Note that the reduced staining in I is due to a reduction in neural plate staining that is a consequence of mispatterning, rather than a direct effect of the hs:dkk1b transgene. In situ hybridization to gfp (K-N) or axin2 (O-R) in hs:wnt8a. gfp staining is detectable 0.5 hph (L), is significantly reduced at 1 hph (M) and is not detectable by 1.5 hph (N). (O) axin2 staining is similar in hs:wnt8a embryos and wild-type siblings. (wt/Tg: genotype of embryos cannot be distinguished by staining). (P) axin2 is strongly induced at 0.5hph (Tg: hs:wnt8a embryo, wt: wild-type sibling; representative embryos shown). (Q) axin2 remains elevated at 1 hph. (R) axin2 levels are indistinguishable between hs:wnt8a and wild-type siblings. |
Reprinted from Developmental Biology, 462(2), Green, D.G., Whitener, A.E., Mohanty, S., Mistretta, B., Gunaratne, P., Yeh, A.T., Lekven, A.C., Wnt signaling regulates neural plate patterning in distinct temporal phases with dynamic transcriptional outputs, 152-164, Copyright (2020) with permission from Elsevier. Full text @ Dev. Biol.