Fig. 4
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- ZDB-FIG-200604-10
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- Green et al., 2020 - Wnt signaling regulates neural plate patterning in distinct temporal phases with dynamic transcriptional outputs
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Timed global Wnt/β-catenin activation reveals two distinct windows of Wnt response. (A) Timeline (hours post fertilization) indicating periods of hs:wnt8a activation. Dashed lines represent uncertainty of beginning and end of Wnt pathway activation after heat shock. C,D,E,F: heat shock activation periods corresponding to images in bottom half of figure. (B–F”) Lateral views of heads of 2427 hpf embryos, anterior left. (B–F) In situ hybridization to zic1. (B’-F’) otx2b. (B”-F”) egr2b. Control: +/+ siblings of hs:wnt8a/+ embryos. Note absence of telencephalic zic1 domain (B, arrow) in all hs:wnt8a/+ embryos. Diencephalic zic1 domain is absent after 4.6 and 6 hpf heat shocks (B,E,F, arrowheads). otx2b expression is suppressed after 4.6 and 6 hpf heat shocks but is present, but shifted anteriorly, after 8 hpf and 9 hpf heat shocks (C’-F’). egr2b expression within r3 and r5 are shifted anteriorly in all treatments with the degree of anterior shift decreasing with later heat shocks (B”-F”, arrows). |
Reprinted from Developmental Biology, 462(2), Green, D.G., Whitener, A.E., Mohanty, S., Mistretta, B., Gunaratne, P., Yeh, A.T., Lekven, A.C., Wnt signaling regulates neural plate patterning in distinct temporal phases with dynamic transcriptional outputs, 152-164, Copyright (2020) with permission from Elsevier. Full text @ Dev. Biol.