Fig. 6
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- ZDB-FIG-200604-8
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- Green et al., 2020 - Wnt signaling regulates neural plate patterning in distinct temporal phases with dynamic transcriptional outputs
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otx2b is directly suppressed by Wnt signaling. (A–H) BIO treatment recapitulates hs:wnt8a effects. In situ hybridization to dmbx1a in midbrain (A–D), egr2b in hindbrain (E–H). Insets in B,C,D show otx2b staining. 27hpf embryos anterior left, dorsal up. Note suppression of midbrain dmbx1a and otx2b after BIO treatment at 6 hpf (B) that is not observed with 8 or 9 hpf treatment (C,D). egr2b is shifted anteriorly, with the degree of anterior shift correlated with time of treatment (E-H; dotted line indicates anterior edge of embryo in H). (I–R) BIO treatment suppresses otx2b in absence of new protein synthesis. Analysis of 10 hpf embryos, oblique dorsal views, animal pole up, in situ hybridizations to otx2b (I–M) or gbx1 (N–R). (J,K,O,P) CHX treatment only. (L,M,Q,R) BIO + CHX treatment. Note the lack of neural plate otx2b after treatment with BIO + CHX at 6 hpf (L), similar to treatment with BIO alone (inset in L), which is not observed after treatment at 8 hpf (M; inset: BIO alone). Remaining mesodermal otx2b positive cells are visible, though numbers vary between embryos. gbx1 is not significantly affected with either CHX or CHX/BIO treatment (N–R). |
Reprinted from Developmental Biology, 462(2), Green, D.G., Whitener, A.E., Mohanty, S., Mistretta, B., Gunaratne, P., Yeh, A.T., Lekven, A.C., Wnt signaling regulates neural plate patterning in distinct temporal phases with dynamic transcriptional outputs, 152-164, Copyright (2020) with permission from Elsevier. Full text @ Dev. Biol.