FIGURE

Fig. 6

ID

ZDB-FIG-200421-9

Publication

Liu et al., 2020 - Zebrafish Pax1a and Pax1b are required for pharyngeal pouch morphogenesis and ceratobranchial cartilage development

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Fig. 6

pax1a- and pax1b-deficient embryos display decreased or absent dlx2a and hand2 expression in pharyngeal arches. Reduced expression of dlx2a (D) or hand2 (I) as well as absence of dlx2a (E) or hand2 (J) in the posterior pharyngeal arches (pa) was identified in 0.75 ng pax1a MO-injected pax1b−/− mutant embryos compared to uninjected pax1b−/− mutant (C, H), wild types (WT; A, F) or 0.75 ng pax1a MO-injected wild types (B, G) at 36 hpf. Similar defects in expression of dlx2a (L) or hand2 (N) in the posterior pharyngeal arches were also identified in pax1a−/−; pax1b−/− double mutants compared with sibling controls (i.e., pax1a+/−; pax1b−/− or pax1a+/+; pax1b−/−) (K, M) at 36 hpf. Asterisks indicate reduced or absent expression. Affected embryos per total number of analyzed embryos are shown. Scale bars represent 100 μm.

Expression Data

Genes:	dlx2a hand2
Fish:	WT pax1b^as37/as37 WT + MO1-pax1a pax1b^as37/as37 + MO1-pax1a pax1a^as36/as36; pax1b^as37/as37
Knockdown Reagent:	MO1-pax1a
Anatomical Terms:	pharyngeal arch 1 pharyngeal arch 2 pharyngeal arch 3 pharyngeal arch 4 pharyngeal arch 5 pharyngeal arch 6
Stage:	Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	pax1b^as37/as37 + MO1-pax1a pax1a^as36/as36; pax1b^as37/as37
Knockdown Reagent:	MO1-pax1a
Observed In:	pharyngeal arch 3 pharyngeal arch 4 pharyngeal arch 5 pharyngeal arch 6
Stage:	Prim-25

Phenotype Detail

Acknowledgments

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Reprinted from Mechanisms of Development, 161, Liu, Y.H., Lin, T.C., Hwang, S.L., Zebrafish Pax1a and Pax1b are required for pharyngeal pouch morphogenesis and ceratobranchial cartilage development, 103598, Copyright (2020) with permission from Elsevier. Full text @ Mech. Dev.