FIGURE

Fig. 8

ID

ZDB-FIG-200421-11

Publication

Liu et al., 2020 - Zebrafish Pax1a and Pax1b are required for pharyngeal pouch morphogenesis and ceratobranchial cartilage development

Other Figures

All Figure Page

Back to All Figure Page

Fig. 8

Similar levels of apoptosis and proliferation were identified in pharyngeal arches 3–4 of wild types and pax1a- and pax1b-deficienct embryos. TUNEL assay was performed in wild types (WT; A, B) and pax1a−/−; pax1b−/− double mutant (C, D) embryos that were hybridized with dlx2a antisense RNA at 26 hpf. Quantification of apoptotic cells in pharyngeal arches (pa) 3 and 4 in wild types and mutants is shown (E). Proliferation was evaluated by anti-PCNA antibody in wild types (F, G) and pax1a−/−; pax1b−/− double mutant (H, I) embryos that were hybridized with dlx2a antisense RNA at 26 hpf. Quantification of proliferation rate in pharyngeal arches 3 and 4 in wild types and mutants is shown (J). Arrowheads indicate neural crest cells labeled with PCNA and dlx2a. Statistical significance was evaluated by Student's t-test, NS, not significant. Error bars indicate standard error. Scale bar represents 100 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	pax1a^as36/as36; pax1b^as37/as37
Observed In:	pharyngeal arch apoptotic process pharyngeal arch cell population proliferation
Stage:	Prim-5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Mechanisms of Development, 161, Liu, Y.H., Lin, T.C., Hwang, S.L., Zebrafish Pax1a and Pax1b are required for pharyngeal pouch morphogenesis and ceratobranchial cartilage development, 103598, Copyright (2020) with permission from Elsevier. Full text @ Mech. Dev.