FIGURE

Figure 2

ID

ZDB-FIG-200129-22

Publication

Cen et al., 2019 - Exosomal Thrombospondin-1 Disrupts the Integrity of Endothelial Intercellular Junctions to Facilitate Breast Cancer Cell Metastasis

Other Figures

All Figure Page

Back to All Figure Page

Figure 2

Exosomes enhance the transendothelial migration of breast cancer cells and inhibit the HUVEC tube formation. (A) Expression of exosome markers in MDA-MB-231 exosomes revealed by Western blot analysis. (B) An electron microscopic image of exosomes (red arrows) derived from MDA-MB-231. Scale bar, 100 nm. (C) Granularity and uniformity of exosomes determined by nanoparticle tracking analysis. (D) Images of transendothelial MDA-MB-231 cells after treating HUVECs with different concentrations of exosomes. Scale bar, 100 μm. (E) Migration of MDA-MB-231 through HUVECs layers after treatment with MDA-MB-231-derived exosomes. (F) Image of tube formation in exosome-treated HUVECs. Scale bar, 1 mm. (G) The tube formation in HUVECs treated with different concentrations of exosomes. (H) Time course of the tube formation in HUVECs treated with 100 μg/mL exosomes. Data are shown as means ± standard deviation (SD) and results were from three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001; ns, no significance by unpaired Student’s t-test.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cancers