FIGURE

Fig. 1

ID
ZDB-FIG-191021-1
Publication
Callahan et al., 2019 - Spinal V2b neurons reveal a role for ipsilateral inhibition in speed control
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Fig. 1

V2b neurons are found throughout the rostral-caudal axis of zebrafish spinal cord.

(A) Transmitted DIC image (top) and confocal image (bottom) of a 5 dpf Tg(gata3:loxP-dsRed-loxP:GFP) animal. Scale bars = 0.5 mm. (B) Lateral view of a midbody spinal cord hemisegment; dashed lines mark muscle segments. In this and all subsequent figures, rostral is to the left and dorsal is to the top. Purple triangles mark CSF-cN neurons and orange stars mark ISN. Scale bar = 20 μm. (C) V2b cell counts per hemisegment quantified along the rostrocaudal body axis, n = 7 fish from three clutches. (D) Example cell morphology using two techniques to label single V2b axons: single-cell dye electroporation (top) and Kaede photoconversion (bottom). Scale bar = 20 μm. (E) Midbody V2b neurons extend axons through more segments than V2b neurons in other rostrocaudal locations. *p<0.01 (p=3.17×10−3); **p<0.001 (p=4.44×10−4). (F) Ball and stick plots indicate soma position and axon extension along the body axis for 35 V2b neurons in 12 animals from two clutches.

Expression Data
Genes:
Fish:
Anatomical Terms:
Stage: Day 5

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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