ZFIN ID: ZDB-FIG-190820-9
Fontenas et al., 2019 - The Neuromodulator Adenosine Regulates Oligodendrocyte Migration at Motor Exit Point Transition Zones. Cell Reports   27:115-128.e5 Full text @ Cell Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Condition:
Anatomical Terms:
Stage Range: Protruding-mouth to Day 4
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: Protruding-mouth to Day 4

Fig. 6

Peripheral OPCs Myelinate Spinal Motor Axons in gpr126Mutant Larvae

(A) wif1 mRNA expression in WT and gpr126−/− larvae at 3 dpf shows MEP glia (arrowheads). SC, spinal cord; N, notochord.

(B) Live images of photoconverted sox10:eos larvae at 3 dpf show Eos+MEP glia (arrowheads) in both WT and gpr126−/− larvae.

(C) Mean ± SEM of peripheral OPCs in 3 dpf gpr126−/− larvae treated with DMSO or SCH-58261 from 36 hpf to 3 dpf. n = 8 DMSO, n = 6 SCH-58261. ∗∗p = 0.009.

(D) Frames from a 15 h time-lapse movie of a olig2:dsred;nkx2.2a:megfp;gpr126−/− larva treated with SCH-58261 from 36 hpf to 3 dpf; 0′ is 57 hpf. Arrowheads mark a nkx2.2a+/olig2+ OPC ensheathing motor axons.

(E) Images of MBP antibody (arrowheads) on peripheral nerves of 4 dpf olig2:dsred;gpr126−/− larvae treated with DMSO or SCH-58261 from 36 hpf to 3 dpf. Asterisk marks peripheral OPC.

(F) Live images of 4 dpf WT and gpr126−/−;olig2:dsred;mbp:egfp larvae treated with DMSO or SCH-58261 from 36 hpf to 3 dpf. Asterisk marks olig2+ peripheral OPC with mbp+ membrane sheaths (arrowheads) around peripheral motor axons. Scale bars, 20 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed2 adgrg6st49/st49; vu19Tg control Day 4 oligodendrocyte IHC
adgrg6st49/st49; vu19Tg chemical treatment by environment: adenosine A2A receptor antagonist Day 4 peripheral nervous system oligodendrocyte IHC
EGFP adgrg6st49/st49; ue2Tg; vu19Tg control Day 4 peripheral neuron myelin sheath IHC
adgrg6st49/st49; ue2Tg; vu19Tg chemical treatment by environment: adenosine A2A receptor antagonist Day 4 peripheral neuron myelin sheath IHC
wif1 AB control Protruding-mouth spinal nerve root glial cell ISH
adgrg6st49/st49 control Protruding-mouth spinal nerve root glial cell ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-mbp IHC adgrg6st49/st49; vu19Tg control Day 4 peripheral neuron myelin sheath
IHC adgrg6st49/st49; vu19Tg chemical treatment by environment: adenosine A2A receptor antagonist Day 4 peripheral neuron myelin sheath
Phenotype Details
Fish Conditions Stage Phenotype
adgrg6st49/st49; ue2Tg; vu19Tg chemical treatment by environment: adenosine A2A receptor antagonist Day 4 peripheral neuron myelin sheath EGFP expression increased distribution, abnormal
Day 4 peripheral neuron myelination increased occurrence, abnormal
adgrg6st49/st49; vu19Tg chemical treatment by environment: adenosine A2A receptor antagonist Protruding-mouth oligodendrocyte displaced to motor neuron axon, abnormal
Protruding-mouth oligodendrocyte displaced to peripheral nervous system, abnormal
Protruding-mouth peripheral nervous system oligodendrocyte increased amount, abnormal
Protruding-mouth peripheral nervous system oligodendrocyte mislocalised, abnormal
Day 4 peripheral nervous system oligodendrocyte DsRed2 expression mislocalised, abnormal
Day 4 peripheral nervous system oligodendrocyte mislocalised, abnormal
Day 4 peripheral neuron myelin sheath ab1-mbp labeling increased distribution, abnormal
Day 4 peripheral neuron myelination increased occurrence, abnormal
Acknowledgments:
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