ZFIN ID: ZDB-FIG-190820-14
Fontenas et al., 2019 - The Neuromodulator Adenosine Regulates Oligodendrocyte Migration at Motor Exit Point Transition Zones. Cell Reports   27:115-128.e5 Full text @ Cell Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Protruding-mouth

Fig. 1

Small-Molecule Screen Identifies Compounds that Disrupt OPC Migration at the MEP TZ

(A) Schematic of a transverse view of the MEP TZ. MEP glia (MEPg; green) prevent OPC (yellow) exit from the spinal cord (gray). OPC processes (arrow) contact MEP glia but are repelled. Without MEP glia, OPCs migrate onto peripheral nerves.

(B) Low-magnification images showing lateral views of negative (top) and positive (bottom) controls at 3 dpf in olig2:dsred larvae. Peripheral olig2+ cells are denoted with yellow arrowheads.

(C) Images of control and validated hits in 3 dpf olig2:dsred larvae showing ectopic OPCs (arrowhead) along spinal motor nerves.

(D) Mean ± SEM of peripheral OPC count for larvae injected with injection buffer (control) or TeNT mRNA at 3 dpf. p = 0.0013; n = 14 control, n = 23 TeNT.

Scale bars, 100 μM in (B) and 20 μM in (C).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed2 vu19Tg control Protruding-mouth oligodendrocyte IFL
vu19Tg chemical treatment by environment: tyrphostin AG 1478 Protruding-mouth oligodendrocyte IFL
Protruding-mouth peripheral nervous system oligodendrocyte IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
vu19Tg chemical treatment by environment: tyrphostin AG 1478 Protruding-mouth oligodendrocyte displaced to motor neuron axon, abnormal
Protruding-mouth oligodendrocyte displaced to peripheral nervous system, abnormal
Protruding-mouth peripheral nervous system oligodendrocyte DsRed2 expression mislocalised, abnormal
Protruding-mouth peripheral nervous system oligodendrocyte mislocalised, abnormal
Acknowledgments:
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