ZFIN ID: ZDB-FIG-190820-13
Fontenas et al., 2019 - The Neuromodulator Adenosine Regulates Oligodendrocyte Migration at Motor Exit Point Transition Zones. Cell Reports   27:115-128.e5 Full text @ Cell Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage Range: Long-pec to Protruding-mouth
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Fig. 2

 A2a AR Antagonist Causes OPC Migration through the MEP TZ

(A) Frames captured from a 15 h time-lapse movie of a sox10:eos;olig2:dsred larva treated with CGS-15943 from 24 hpf to 3 dpf and imaged from 57 to 72 hpf; 0′ is 58 hpf. The black asterisk marks OPC body. Arrowheads mark OPC leading process.

(B) Dose-response curve of the number of peripheral OPCs for fish treated from 24 hpf to 3 dpf with SCH-58261. Mean ± SEM, n = 10 larvae per dose; ∗∗p < 0.01 and ∗∗∗p < 0.0001.

(C) Percentage of nerves at 3 dpf that have ectopic OPC processes and/or cell bodies. n = 60 DMSO, n = 98 SCH-58261; p < 0.01.

(D) Mean ± SEM of peripheral OPCs in olig2:dsred larvae treated with 10 μM SCH-58261 alone or in combination with 2.5 μM CGS-21680 or 5 μM adenosine from 36 hpf to 3 dpf. n = 30 fish per treatment; p < 0.05 and ∗∗p < 0.01.

(E) Peripheral OPC counts for larvae treated with 10 μM SCH-58261 during distinct time periods. Green bars indicate mean peripheral OPC counts significantly above DMSO control (p < 0.05). Red bars indicate mean peripheral OPC counts not significantly different than DMSO (p > 0.05). n = 9–12 larvae per condition. Scale bar, 20 μM.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed2 vu19Tg; w9Tg chemical treatment by environment: CGS 15943 Long-pec to Protruding-mouth oligodendrocyte IFL
Long-pec to Protruding-mouth peripheral nervous system oligodendrocyte IFL
Eos vu19Tg; w9Tg chemical treatment by environment: CGS 15943 Long-pec to Protruding-mouth oligodendrocyte IFL
Long-pec to Protruding-mouth peripheral nervous system oligodendrocyte IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
vu19Tg; w9Tg chemical treatment by environment: CGS 15943 Long-pec - Protruding-mouth oligodendrocyte glial cell migration process quality, abnormal
Long-pec - Protruding-mouth peripheral nervous system oligodendrocyte DsRed2 expression mislocalised, abnormal
Long-pec - Protruding-mouth peripheral nervous system oligodendrocyte Eos expression mislocalised, abnormal
Protruding-mouth oligodendrocyte displaced to motor neuron axon, abnormal
Protruding-mouth oligodendrocyte displaced to peripheral nervous system, abnormal
Protruding-mouth peripheral nervous system oligodendrocyte increased amount, abnormal
Protruding-mouth peripheral nervous system oligodendrocyte mislocalised, abnormal
vu19Tg; w9Tg chemical treatment by environment: adenosine A2A receptor antagonist Long-pec - Protruding-mouth oligodendrocyte glial cell migration process quality, abnormal
Protruding-mouth oligodendrocyte displaced to motor neuron axon, abnormal
Protruding-mouth oligodendrocyte displaced to peripheral nervous system, abnormal
Protruding-mouth peripheral nervous system oligodendrocyte increased amount, abnormal
Protruding-mouth peripheral nervous system oligodendrocyte mislocalised, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Rep.