Figure 5—figure supplement 1.

<italic>klf2a</italic> and <italic>trpv4</italic> mutants display reduced endocardial Notch signaling.

(A–C) Confocal microscopy imaging performed on vmhc:mCherry-NTR, Tp1:d2GFP control hearts reveals reduced Tp1:d2GFP activation in (B) klf2a-/- and (C) trpv4-/- hearts at 24 hpt (6 dpf) when compared to (A) wild-type (wt) hearts (n = 15 control wt; 18 control trpv4-/-; 20 control klf2a-/-; 22 MTZ wt; 21 MTZ trpv4-/-; 18 MTZ klf2a-/-). (D–F) Whole-mount in situ hybridizations reveal reduced gene expression of notch1b in (E) klf2a-/- (n = 4/19) and (F) trpv4-/- (n = 14/29) vmhc:mCherry-NTR control hearts at 24 hpt (6 dpf) when compared to (D) wild-type (wt) hearts (n = 22/25). All confocal images shown are maximum intensity projections. V, ventricle; A, atrium; dpf, days post-fertilization; hpt, hours post-MTZ/DMSO treatment. Dashed lines outline the heart. Bars: 50 μm.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife