ZFIN ID: ZDB-FIG-190723-1025
Gálvez-Santisteban et al., 2019 - Hemodynamic-mediated endocardial signaling controls in vivo myocardial reprogramming. eLIFE   8 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Conditions:
Anatomical Term:
Stage: Day 6
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage: Day 6

Figure 3—figure supplement 1.

Inhibiting hemodynamic flow leads to reduced cardiac Klf2a and Notch signaling.

( A–D) Confocal imaging performed on  klf2a:H2B-GFP ; vmhc:mCherry-NTR uninjured hearts at 24 hpt (6 dpf) reveals decreased  klf2a:H2B-GFP expression in ( B) blebbistatin (Blebb) and ( C) tricaine (Tric) treated hearts as well as in ( Dgata2a-/- mutant hearts when compared to ( A) ethanol (EtOH)-treated control hearts (n = 12 control EtOh; 6 control Blebb; 7 control Tric; 9 control  gata2a-/-). ( E–H) Confocal imaging performed on  Tp1:d2GFP ; vmhc:mCherry-NTR uninjured hearts at 24 hpt (6 dpf) shows decreased  Tp1:d2GFP Notch reporter expression in hearts treated with ( F) blebbistatin or ( G) tricaine when compared to ( E) ethanol-treated control hearts; however, ( Hgata2a-/-hearts do not display significant changes as quantitatively assessed in  Figure 3N(n = 12 control EtOh; 5 control Blebb; 7 control Tric; 9 control  gata2a-/-). ( I–L) Whole-mount in situ hybridizations reveal decreased  notch1b expression in hearts treated with ( J) blebbistatin (n = 0/9) or ( K) tricaine (n = 0/5) and in ( Lgata2a-/- hearts (n = 4/12) when compared to ( I) ethanol-treated control hearts (n = 10/10) at 24 hpt (6 dpf). All confocal images shown are maximum intensity projections. V, ventricle; A, atrium; dpf, days post-fertilization; hpt, hours post-DMSO treatment. Dashed lines outline the heart. Bars: 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
d2EGFP mw43Tg ; s957Tg chemical treatment by environment: blebbistatin Day 6 heart IFL
mw43Tg ; s957Tg chemical treatment by environment: tricaine Day 6 heart IFL
mw43Tg ; s957Tg ; gata2aum27/um27 control Day 6 heart IFL
EGFP ig11Tg ; s957Tg chemical treatment by environment: blebbistatin Day 6 heart IFL
ig11Tg ; s957Tg chemical treatment by environment: tricaine Day 6 heart IFL
ig11Tg ; s957Tg ; gata2aum27/um27 control Day 6 heart IFL
notch1b gata2aum27/um27 ; s957Tg control Day 6 heart ISH
s957Tg chemical treatment by environment: blebbistatin Day 6 heart ISH
s957Tg chemical treatment by environment: tricaine Day 6 heart ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ig11Tg ; s957Tg chemical treatment by environment: blebbistatin Day 6 heart EGFP expression decreased amount, abnormal
ig11Tg ; s957Tg chemical treatment by environment: tricaine Day 6 heart EGFP expression decreased amount, abnormal
mw43Tg ; s957Tg chemical treatment by environment: blebbistatin Day 6 heart d2EGFP expression decreased amount, abnormal
mw43Tg ; s957Tg chemical treatment by environment: tricaine Day 6 heart d2EGFP expression decreased amount, abnormal
s957Tg chemical treatment by environment: blebbistatin Day 6 heart notch1b expression decreased amount, abnormal
s957Tg chemical treatment by environment: tricaine Day 6 heart notch1b expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife