Fig. 3

(A-D) Lateral views of dissected adult wild type and taz^-/- ovaries. Similar to wild type (A), the taz mutant ovary (B) is grossly normal, and taz mutant oocytes (D) are similar to wild type (C) in size and numbers. (E-F) Haematoxylin and eosin (HE) stained sections show no apparent morphological difference in oogenesis between wild type (E) and taz^-/-ovaries (F). Oocytes at stages I-IV are marked; n, nucleus; green box, CG (cortical granules). (G-J) In situ hybridization on sectioned ovaries to detect oocyte polarity markers show that animal-vegetal polarity is established in taz^-/- oocytes similar to wild type oocytes. The Balbiani body, and subsequently, vegetal pole labeled by expression of dazl in wild type and taz^-/- are normal in primary (G, H) and stage III (I, J) oocytes, and the animal pole, marked by cyclinB transcripts, is properly established in both wild type and taz^-/-oocytes (I, J). Dark blue arrowhead, Balbiani body; dark blue arrow, vegetal pole; pink arrow, animal pole. Scale bar, 1 mm in A-D, 100 μm in E-F, and 50 μm in G-J.