PUBLICATION

The effector of Hippo signaling, Taz, is required for formation of the micropyle and fertilization in zebrafish

Authors

Yi, X., Yu, J., Ma, C., Dong, G., Shi, W., Li, H., Li, L., Luo, L., Sampath, K., Ruan, H., Huang, H.

ID

ZDB-PUB-190105-6

Date

2019

Source

PLoS Genetics 15: e1007408 (Journal)

Registered Authors

Dong, Guoping, Huang, Honghui, Li, Li, Luo, Lingfei, Ruan, Hua, Sampath, Karuna, Yi, Xaogui

Keywords

none

MeSH Terms

Embryo, Nonmammalian
Embryonic Development/genetics*
Oogenesis/genetics*
Zebrafish Proteins/genetics*
Female
Spermatozoa/growth & development
Spermatozoa/ultrastructure
Cytoskeleton/genetics
Cytoskeleton/ultrastructure
Oocytes/growth & development
Protein Serine-Threonine Kinases/genetics
Fertilization/genetics*
Signal Transduction
Transcription Factors/genetics*
Ovary/growth & development
Ovary/ultrastructure
Animals, Genetically Modified
Male
Zebrafish/genetics
Zebrafish/growth & development
Animals

(all 21)

PubMed

30608921 Full text @ PLoS Genet.

Abstract

The mechanisms that ensure fertilization of egg by a sperm are not fully understood. In all teleosts, a channel called the 'micropyle' is the only route of entry for sperm to enter and fertilize the egg. The micropyle forms by penetration of the vitelline envelope by a single specialized follicle cell, the micropylar cell. The mechanisms underlying micropylar cell specification and micropyle formation are poorly understood. Here, we show that an effector of the Hippo signaling pathway, the Transcriptional co-activator with a PDZ-binding domain (Taz), plays crucial roles in micropyle formation and fertilization in zebrafish (Danio rerio). Genome editing mutants affecting taz can grow to adults. However, eggs from homozygous taz females are not fertilized even though oocytes in mutant females are histologically normal with intact animal-vegetal polarity, complete meiosis and proper ovulation. We find that taz mutant eggs have no micropyle. Taz protein is specifically enriched in mid-oogenesis in the micropylar cell located at the animal pole of wild type oocyte, where it might regulate the cytoskeleton. Taz protein and micropylar cells are not detected in taz mutant ovaries. Our work identifies a novel role for the Hippo/Taz pathway in micropylar cell specification in zebrafish, and uncovers the molecular basis of micropyle formation in teleosts.

Genes / Markers

Figures

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Expression

Phenotype

Fish	Conditions	Stage	Phenotype	Figure
wwtr1^swu46/+ (AB/TU)	standard conditions	1-cell	chorion micropyle formation decreased occurrence, abnormal	Fig. 4
wwtr1^swu46/+ (AB/TU)	standard conditions	1-cell	fertilization decreased occurrence, abnormal	Fig. 4
wwtr1^swu46/+ (AB/TU)	standard conditions	1-cell	fertilized egg chrorion micropyle absent, abnormal	Fig. 4
wwtr1^swu46/+ (AB/TU)	standard conditions	1-cell	fertilized egg pronucleus decreased amount, abnormal	Fig. 4
wwtr1^swu46/+ (AB/TU)	standard conditions	1-cell to 8-cell	embryo development arrested, abnormal	Fig. 2
wwtr1^swu46/swu46 (AB/TU)	standard conditions	Day 4	pericardium edematous, abnormal	Fig. 1
wwtr1^swu46/swu46 (AB/TU)	standard conditions	Day 4	swim bladder decreased size, abnormal	Fig. 1
wwtr1^swu46/swu46 (AB/TU)	standard conditions	Day 5	whole organism wwtr1 expression absent, abnormal	Fig. 1
wwtr1^swu46/swu46 (AB/TU)	standard conditions	Adult	chorion micropyle formation decreased occurrence, abnormal	Fig. 5
wwtr1^swu46/swu46 (AB/TU)	standard conditions	Adult	micropylar cell wwtr1 expression absent, abnormal	Fig. 5

1 - 10 of 14

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Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
swu46		Small Deletion	wwtr1
swu47		Small Deletion	wwtr1

Human Disease / Model

Sequence Targeting Reagents

Fish

Fish
AB/TU
wwtr1^swu46/+ (AB/TU)
wwtr1^swu46/swu46 (AB/TU)
wwtr1^swu47/+ (AB/TU)

Antibodies

Orthology

Engineered Foreign Genes

Mapping

Yi et al., 2019 ZDB-PUB-190105-6 PMID:30608921

The effector of Hippo signaling, Taz, is required for formation of the micropyle and fertilization in zebrafish

Yi et al., 2019

ZDB-PUB-190105-6
PMID:30608921