FIGURE

Fig. 1

ID
ZDB-FIG-190606-10
Publication
Yi et al., 2019 - The effector of Hippo signaling, Taz, is required for formation of the micropyle and fertilization in zebrafish
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Fig. 1

Some homozygous taz mutants can survive to adulthood.

(A-C) Schematic of CRISPR/Cas9 knock-out of taz. The target site (shown in blue) is located in exon 1 of taz, and the PAM region is shown in red (A). Two mutant alleles with small deletions, taz Δ10 and taz Δ1, were obtained (B), both of which result in frame-shifts of the taz open reading frame and truncated proteins (C). aa, amino acid; TEAD BD, Tead transcription factor binding domain (light blue); WW, dual tryptophan motif (green); TAD, transactivation domain (purple); PDZ domain (blue). (D) Western blot analysis shows Taz protein in wild type but not in tazΔ10/ Δ10 mutant embryos. β-Tubulin is used as a loading control. (E, F) Similar to wild type embryos (E), tazΔ10/Δ10 embryos are largely normal at 4.5 dpf, except for a smaller inflated swim bladder and mild pericardial edema (F). (G) Survival curve of tazΔ10/Δ10 in heterozygote intercrosses from larval to adult stages (shown as % survivors, from two independent experiments). The red dashed line indicates the expected 25% homozygous mutants in accordance with Mendelian segregation. Number of larvae/adults at each stage: 7 dpf (59/228), 15 dpf (37/200), 30 dpf (20/182), 60 dpf (22/216) and 120 dpf (10/174). Black arrow, swim bladder; black arrowhead, pericardium. Scale bar, 500 μm.

Expression Data
Gene:
Fish:
Stage: Day 5

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Observed In:
Stage Range: Day 4 to Day 5

Phenotype Detail
Acknowledgments
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