FIGURE

Fig. S2

ID

ZDB-FIG-180906-74

Publication

Frétaud et al., 2017 - High-resolution 3D imaging of whole organ after clearing: taking a new look at the zebrafish testis

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Fig. S2

Confocal imaging of transverse vibratome sections of CUBIC-‐ cleared and uncleared testes.

Testes were dissected from the zebrafish transgenic line Tg(gsdf:GFP), cleared or not with CUBIC and stained with propidium iodide. Transverse sections were performed with a vibratome to assess the nuclear staining in depth. Confocal images show that all nuclei in the center of the sample are efficiently stained. Images correspond to maximal projections of 10 optical slices (z-‐step = 3 μm). Nuclei are in magenta and Sertoli GFP cells in green. Scale bar: 100 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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