FIGURE

Fig. 5

ID

ZDB-FIG-180906-69

Publication

Frétaud et al., 2017 - High-resolution 3D imaging of whole organ after clearing: taking a new look at the zebrafish testis

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

(a) Optical section of a non-cleared testis. Nuclei were stained with propidium iodide. Nuclei of germ cells at different stages of differentiation are packed in distinct domains. A spermatozoa domain is delineated in blue and a primary spermatocyte domain is delineated in orange. For each clearing condition (RIMS, 3DISCO, CUBIC and PACT), the nuclear area of 55 spermatozoa and 55 primary spermatocytes was measured, spreading across 11 optical sections from two testes. (b) Nuclear area of spermatozoa. (c) Nuclear area of primary spermatocytes. All data are indicated as mean ± SD. *Indicates a significant difference as compared with PBS (p < 0.0001) using a one way ANOVA with Dunnet’s post-hoc test for multiple comparison. Scale bar: 25 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.