FIGURE

Fig. 2

ID

ZDB-FIG-180906-66

Publication

Frétaud et al., 2017 - High-resolution 3D imaging of whole organ after clearing: taking a new look at the zebrafish testis

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Fig. 2

Two-photon imaging of testes cleared with RIMS, SeeDB, 3DISCO, CUBIC or PACT protocols. (a) XZ planes of testes. Nuclei were stained with propidium iodide (in magenta). Laser intensity was set in order to be next to saturation at the beginning of the stack and no depth compensation was used. No brightness and contrast enhancement was applied. (b) Quantification of fluorescence intensity. Mean fluorescence intensity was normalized and plotted against the imaging depth. Mean ± SEM of 4–12 ROI acquired on 2–3 different testes.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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