ZFIN ID: ZDB-FIG-180823-8
Moreno et al., 2018 - Investigation of Islet2a function in zebrafish embryos: Mutants and morphants differ in morphologic phenotypes and gene expression. PLoS One   13:e0199233 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Prim-5

Fig. 4

Islet2a morphant but not mutant embryos displayed altered CaP morphology.

(A-E) In live tg(mnx1:gfp) 24 hpf embryos, CaP neurons expressed gfp in their somas and axons. (A, B) Injection of a T-MO (MO) led to truncation of ventrally projecting axons (asterisks, B) compared to control (Ctl, A), as previously reported [15, 17]. (C-E) In wildtype (wt, C), heterozygous (het, D) and mutant (mut, E) islet2a embryos, CaP neuron axon growth and trajectories appeared normal regardless of genotype. Sample size ranged from 8–30 per condition. Scale bar in E, for A-E: 50 μm. (F, G) In fixed non-transgenic 28 hpf embryos, zn1/znp1 immunoreactivity did not reveal any differences in CaP axon morphology between wildtype (F; n = 9) versus mutant (G; n = 9) embryos.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP isl2aco7002/+; ml2Tg standard conditions Prim-5 secondary motor neuron axon IFL
Prim-5 secondary motor neuron neuronal cell body IFL
isl2aco7002/co7002; ml2Tg standard conditions Prim-5 secondary motor neuron axon IFL
Prim-5 secondary motor neuron neuronal cell body IFL
ml2Tg control Prim-5 CaP motoneuron axon IFL
Prim-5 CaP motoneuron neuronal cell body IFL
ml2Tg + MO1-isl2a standard conditions Prim-5 CaP motoneuron axon IFL
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
zn-1 IHC WT standard conditions Prim-5 CaP motoneuron
IHC isl2aco7002/co7002 standard conditions Prim-5 CaP motoneuron
znp-1 IHC WT standard conditions Prim-5 CaP motoneuron
IHC isl2aco7002/co7002 standard conditions Prim-5 CaP motoneuron
Phenotype Details
Fish Conditions Stage Phenotype
isl2aco7002/+; ml2Tg standard conditions Prim-5 CaP motoneuron morphology, normal
isl2aco7002/co7002 standard conditions Prim-5 CaP motoneuron morphology, normal
isl2aco7002/co7002; ml2Tg standard conditions Prim-5 CaP motoneuron morphology, normal
ml2Tg + MO1-isl2a standard conditions Prim-5 CaP motoneuron axon GFP expression decreased amount, abnormal
Prim-5 CaP motoneuron axon morphology, abnormal
Prim-5 CaP motoneuron axon truncated, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One