FIGURE

Fig. 4

ID

ZDB-FIG-180823-8

Publication

Moreno et al., 2018 - Investigation of Islet2a function in zebrafish embryos: Mutants and morphants differ in morphologic phenotypes and gene expression

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Fig. 4

Islet2a morphant but not mutant embryos displayed altered CaP morphology.

(A-E) In live tg(mnx1:gfp) 24 hpf embryos, CaP neurons expressed gfp in their somas and axons. (A, B) Injection of a T-MO (MO) led to truncation of ventrally projecting axons (asterisks, B) compared to control (Ctl, A), as previously reported [15, 17]. (C-E) In wildtype (wt, C), heterozygous (het, D) and mutant (mut, E) islet2a embryos, CaP neuron axon growth and trajectories appeared normal regardless of genotype. Sample size ranged from 8–30 per condition. Scale bar in E, for A-E: 50 μm. (F, G) In fixed non-transgenic 28 hpf embryos, zn1/znp1 immunoreactivity did not reveal any differences in CaP axon morphology between wildtype (F; n = 9) versus mutant (G; n = 9) embryos.

Expression Data

Gene:	GFP
Antibodies:	zn-1 znp-1
Fish:	WT isl2a^{co7002/co7002} ml2Tg ml2Tg + MO1-isl2a isl2a^co7002/+; ml2Tg isl2a^{co7002/co7002}; ml2Tg
Knockdown Reagent:	MO1-isl2a
Anatomical Terms:	CaP motoneuron CaP motoneuron axon CaP motoneuron neuronal cell body secondary motor neuron axon secondary motor neuron neuronal cell body
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	isl2a^{co7002/co7002} ml2Tg + MO1-isl2a isl2a^co7002/+; ml2Tg isl2a^{co7002/co7002}; ml2Tg
Knockdown Reagent:	MO1-isl2a
Observed In:	CaP motoneuron CaP motoneuron axon
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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