ZFIN ID: ZDB-FIG-180823-11
Moreno et al., 2018 - Investigation of Islet2a function in zebrafish embryos: Mutants and morphants differ in morphologic phenotypes and gene expression. PLoS One   13:e0199233 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Anatomical Term:
Stage: Prim-5
PHENOTYPE:
Fish:
Observed In:
Stage: Prim-5

Fig. 9

CaPs expressed reduced levels of Isl1/2 immunoreactivity in islet2a mutant embryos.

(A) In 28 hpf tg(mnx1:gfp) wildtype (wt, n = 15) embryos, CaPs (*) expressed both gfp (green) and Isl1/2 immunoreactivity (red), as revealed by the merged yellow signal. (B) The Isl11/2 immunosignal of Panel A is shown separately. In A-D, dotted lines circle the cell bodies of CaP/VaPs that were immunopositive for Isl1/2. In comparison to other ventral neurons, CaP Isl1/2 immunolabeling was more intense. (C) In 28 hpf tg(mnx1:gfp) mutant (mut, n = 14) embryos, CaPs (*) expressed gfp. However, compared to wildtype (A), the CaP Isl1/2 fluorescent immunolabel signal was less intense. Further, other ventral neurons continued to display Isl1/2 immunoreactivity. (D) The Isl1/2 signal of Panel C is viewed separately. Compared to wildtype (B), CaPs expressed reduced levels of Isl1/2 immunoreactivity. Further, despite the weak signal in CaPs, Isl1/2 immunoreactivity was present in other ventral neurons at levels similar to wildtype (B). Scale bar in D for A-D: 25 μm. (E, F) Examination of CaP Isl1/2 immunolabeling dorsal to the proctodeum. (E) In wildtype embryos, both the proctodeum (white arrow) and CaPs (asterisks) displayed Isl1/2 immunolabeling. One CaP, contained within white dotted line box, is shown at higher magnification in the inset. (F) In mutant embryos, Isl1/2 immunolabeling was not detected in the proctodeum, consistent with loss of Islet2a protein expression. Despite this, a low level of Isl1/2 immunoreactivity persisted in CaPs (asterisks; one CaP shown at higher power in inset).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP isl2aco7002/+; ml2Tg standard conditions Prim-5 CaP motoneuron IHC
ml2Tg standard conditions Prim-5 CaP motoneuron IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab2-isl IHC isl2aco7002/+; ml2Tg standard conditions Prim-5 CaP motoneuron
IHC ml2Tg standard conditions Prim-5 CaP motoneuron
Phenotype Details
Fish Conditions Stage Phenotype
isl2aco7002/+; ml2Tg standard conditions Prim-5 CaP motoneuron ab2-isl labeling decreased amount, abnormal
Acknowledgments:
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