ZFIN ID: ZDB-FIG-180823-10
Moreno et al., 2018 - Investigation of Islet2a function in zebrafish embryos: Mutants and morphants differ in morphologic phenotypes and gene expression. PLoS One   13:e0199233 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Prim-5

Fig. 8

CaPs expressed islet2a, but not islet1 or islet2b, in wildtypes and mutants.

The expression patterns of islet1 (A [n = 6], B [n = 8], islet2a (C [n = 6], D [n = 8]) and islet2b (E [n = 9], F [n = 8]) in CaPs were examined in 28 hpf sibling wildtype (A, C, E) and islet2a mutant (B, D, F) tg(mnx1:gfp) embryos. At 28 hpf, VaP (variably-present CaP duplicate) is present in some segments. In A-F, white and green asterisks denote lone CaPs and CaP/VaP pairs, respectively. Novel, ectopic expression of either islet1 or islet2b in CaPs was not detected.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
GFP isl2aco7002/+; ml2Tg standard conditions Prim-5 motor neuron IHC
ml2Tg standard conditions Prim-5 motor neuron IHC
isl1a isl2aco7002/+; ml2Tg standard conditions Prim-5 Not Detected CaP motoneuron ISH
ml2Tg standard conditions Prim-5 Not Detected CaP motoneuron ISH
isl2a isl2aco7002/+; ml2Tg standard conditions Prim-5 CaP motoneuron ISH
ml2Tg standard conditions Prim-5 CaP motoneuron ISH
isl2b isl2aco7002/+; ml2Tg standard conditions Prim-5 Not Detected CaP motoneuron ISH
ml2Tg standard conditions Prim-5 Not Detected CaP motoneuron ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
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