Fig. 7

In vivo xenotransplantation assay for different inhibitors or MAN1A1 shRNA treatment in MAN1A1‐overexpressing cells. The cell lines were labeled with a CFSE florescent dye and injected into the yolks of 2‐day‐old zebrafish embryos. (A) Representative images of 1 day postinjection versus 3 days postinjection of MAN1A1 transiently overexpressing Hep3B cells treated with sterile water, DMJ, 4‐PBA, TUDCA, and MAN1A1 shRNA are shown from the same fish at 1 day postinjection and 3 days postinjection. (B) The percentage of embryos with increased, no change, or decreased number of injected CFSE‐dyed cells was analyzed using Image J and compared between 3 days postinjection and 1 day postinjection. (C) The proliferation ability of MAN1A1 transiently overexpressing Hep3B cells was effectively inhibited by the mannosidase inhibitors, ER stress inhibitor, and MAN1A1 shRNA. The data are presented as dot plots with a horizontal line for the mean. Experiments were repeated 3 times. Statistical significance was calculated using the Student t test (*P < 0.05, **P < 0.01, ***P < 0.001). Images were taken at magnification ×48, and the scale shown is for 1 mm. Abbreviation: dpi, dots per inch.