FIGURE

Fig. 2

ID
ZDB-FIG-180516-4
Publication
Zhang et al., 2017 - Rb1 promotes T cell maturation from premature apoptosis by inhibiting E2F1
Other Figures
All Figure Page
Back to All Figure Page
Fig. 2

T-cell maturation is impaired in rb1-deficient embryos. (A) Expression of ikaros, lck and rag1 in the thymus (broken line) of siblings and rb1 mutants at 5 dpf (upper panels). Embryos for whole-mount in situ hybridization were obtained from an incross of genotyped heterozygous rb1 mutants. Scale bars: 100 μm. The positive signal areas were analysed using Image-Pro Plus (lower panel) (***P<0.001; mean±s.e.m.; n=16). (B) Confocal images of rag2:DsRed cells in the thymus of the siblings and rb1smu8/smu8 mutants from 4 dpf to 8 dpf (upper panels). Scale bars: 50 μm. Quantification of T-cell number in sibling embryos and rb1smu8/smu8 mutants (lower panel) (***P<0.001; mean±s.e.m.; n=10). (C) Schematic of the lateral view of a zebrafish indicating the region of the thymus excised for RNA extraction (red dot). qPCR analysis of rag2, cd4, foxn1 and myb in the thymus of siblings and rb1-deficient embryos at 5 dpf (mean±s.e.m.; **P<0.01, *P<0.05, ns, not significant; n=30). (D) Whole-mount in situ hybridization of foxn1 in the thymus (broken line) of siblings and rb1smu8/smu8 mutants at 5 dpf. Scale bars: 100 μm. (E) Whole-mount in situ hybridization of cmyb in the thymus of the siblings and rb1smu8/smu8 mutants at 3 dpf. Scale bars: 20 μm. (F) Whole-mount in situ hybridization of rag1 in the thymus (broken line) at 5 dpf after injecting with control and pTol-lck:rb1 plasmid. Scale bars: 100 μm.

Expression Data
Genes:
Fish:
Anatomical Terms:
Stage Range: Protruding-mouth to Days 7-13

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Observed In:
Stage Range: Day 4 to Days 7-13

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development