FIGURE

Fig. 8

ID

ZDB-FIG-180131-18

Publication

Escobar-Aguirre et al., 2017 - Microtubule-actin crosslinking factor 1 (Macf1) domain function in Balbiani body dissociation and nuclear positioning

Other Figures

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Fig. 8

Macf1 localization and expression in macf1a^p1CH1 and macf1a^p2CH1CH2 mutants.

A-C) Macf1a immunostaining (red) in WT and macf1a^p1CH1/macf1a^sa12708 and macf1a^p2CH1CH2. N≥ 3 ovaries, 20 oocytes macf1a^p1CH1/macf1a^sa12708 and 28 oocytes macf1a^p2CH1CH2. D) AV polarity in WT and lack of polarity in macf1a^sa12708, macf1a^p1CH1/macf1a^sa12708 and macf1a^p2CH1CH2/macf1^sa12708 mutant eggs, where the cytoplasm (arrowheads) surrounds the yolk instead of forming the blastodisc. Mutant macf1a^p1CH1 females produced an incompletely penetrant egg phenotype. Late blastula embryos from macf1a^p3PRD/ macf1^sa12708 and macf1a^p3PRD mutant females displayed normal AV polarity and development. Arrowheads in D indicate the egg cytoplasm. Scale bar: (A-C) 20 μm and (D) 100 μm.

Expression Data

Gene:	macf1a
Fish:	TU macf1a^{p2CH1CH2/p2CH1CH2} macf1a^sa12708/+; macf1a^p1CH1/+
Anatomical Term:	oocyte stage I mitochondrial cloud
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	macf1a^p1CH1/p1CH1 macf1a^{sa12708/sa12708} macf1a^sa12708/+; macf1a^p1CH1/+ macf1a^sa12708/+; macf1a^p2CH1CH2/+ macf1a^sa12708/+; macf1a^p3PRD/+
Observed In:	oocyte stage I oocyte stage I establishment of cell polarity
Stage:	Adult

Phenotype Detail

Acknowledgments

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