ZFIN ID: ZDB-FIG-171206-66
Witzel et al., 2017 - Isl2b regulates anterior second heart field development in zebrafish. Scientific Reports   7:41043 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. 2

Islet family members are expressed in distinct patterns in the developing heart.

(a) Confocal images of control and Tg(myl7:EGFP-HsHRAS)s883isl1−/− embryos or Tg(myl7:EGFP-HsHRAS)s883 isl1−/− embryos following morpholino-mediated knockdown of Isl2a, Isl2b or Isl2a/Isl2b stained with anti-GFP and anti-Isl1/2 antibodies at 26 hpf. (b) Confocal images of Tg(flk1:EGFP) Isl2a/Isl2b morpholino-injected embryo stained with anti-GFP and anti-Isl1/2 antibodies at 26 hpf. Asterisk indicates the late ventricular region22. White arrows indicate Isl1+ cardiomyocytes at the venous pole of the atrium; yellow arrows point to Isl2a+ cells in the pericardial wall; yellow arrowheads point to Isl2a+ cells in the adjacent endoderm; white arrowheads point to Isl2b+ cardiomyocytes at the inner curvature of the ventricle and the outflow pole; blue arrowheads point to Isl1+ endothelial cells. Scale bars in (a,b), 50 μm. (c) Schematic representation of the distinct Isl1+, Isl2a+ and Isl2b+ populations at the linear heart tube stage.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP isl1asa29/sa29; s883Tg standard conditions Prim-5 cardiac muscle cell IHC
isl1asa29/sa29; s883Tg + MO1-isl2a standard conditions Prim-5 cardiac muscle cell IHC
isl1asa29/sa29; s883Tg + MO1-isl2a + MO2-isl2b standard conditions Prim-5 cardiac muscle cell IHC
isl1asa29/sa29; s883Tg + MO2-isl2b standard conditions Prim-5 cardiac muscle cell IHC
s843Tg + MO1-isl2a + MO2-isl2b standard conditions Prim-5 primitive heart tube endothelial cell IHC
s883Tg standard conditions Prim-5 cardiac muscle cell IHC
s883Tg + MO1-isl2a + MO2-isl2b standard conditions Prim-5 cardiac muscle cell IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab2-isl IHC isl1asa29/sa29; s883Tg standard conditions Prim-5 presumptive bulbus arteriosus
IHC Prim-5 presumptive cardiac ventricle heart tube anatomical region
IHC Prim-5 Not Detected presumptive sinus venosus cardiac muscle cell
IHC isl1asa29/sa29; s883Tg + MO1-isl2a standard conditions Prim-5 presumptive bulbus arteriosus
IHC Prim-5 presumptive cardiac ventricle heart tube
IHC Prim-5 presumptive cardiac ventricle heart tube anatomical region
IHC Prim-5 Not Detected presumptive sinus venosus cardiac muscle cell
IHC isl1asa29/sa29; s883Tg + MO1-isl2a + MO2-isl2b standard conditions Prim-5 Not Detected presumptive sinus venosus cardiac muscle cell
IHC isl1asa29/sa29; s883Tg + MO2-isl2b standard conditions Prim-5 pericardium
IHC Prim-5 Not Detected presumptive sinus venosus cardiac muscle cell
IHC s843Tg + MO1-isl2a + MO2-isl2b standard conditions Prim-5 presumptive sinus venosus cardiac muscle cell
IHC s883Tg standard conditions Prim-5 pericardium
IHC Prim-5 presumptive bulbus arteriosus
IHC Prim-5 presumptive cardiac ventricle heart tube
IHC Prim-5 presumptive cardiac ventricle heart tube anatomical region
IHC Prim-5 presumptive sinus venosus cardiac muscle cell
IHC s883Tg + MO1-isl2a + MO2-isl2b standard conditions Prim-5 presumptive sinus venosus cardiac muscle cell
Phenotype Details
Fish Conditions Stage Phenotype
isl1asa29/sa29; s883Tg standard conditions Prim-5 presumptive sinus venosus cardiac muscle cell ab2-isl labeling absent, abnormal
isl1asa29/sa29; s883Tg + MO1-isl2a standard conditions Prim-5 presumptive sinus venosus cardiac muscle cell ab2-isl labeling absent, abnormal
isl1asa29/sa29; s883Tg + MO1-isl2a + MO2-isl2b standard conditions Prim-5 presumptive sinus venosus cardiac muscle cell ab2-isl labeling absent, abnormal
isl1asa29/sa29; s883Tg + MO2-isl2b standard conditions Prim-5 presumptive sinus venosus cardiac muscle cell ab2-isl labeling absent, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.