PUBLICATION

Isl2b regulates anterior second heart field development in zebrafish

Authors
Witzel, H.R., Cheedipudi, S., Gao, R., Stainier, D.Y., Dobreva, G.D.
ID
ZDB-PUB-170121-4
Date
2017
Source
Scientific Reports   7: 41043 (Journal)
Registered Authors
Dobreva, Gergana, Stainier, Didier
Keywords
Differentiation, Morphogenesis
MeSH Terms
  • Animals
  • Gene Expression Regulation, Developmental*
  • Heart/growth & development*
  • LIM-Homeodomain Proteins/metabolism*
  • Myocardium/metabolism
  • Transcription Factors/metabolism*
  • Zebrafish
PubMed
28106108 Full text @ Sci. Rep.
Abstract
After initial formation, the heart tube grows by addition of second heart field progenitor cells to its poles. The transcription factor Isl1 is expressed in the entire second heart field in mouse, and Isl1-deficient mouse embryos show defects in arterial and venous pole development. The expression of Isl1 is conserved in zebrafish cardiac progenitors; however, Isl1 is required for cardiomyocyte differentiation only at the venous pole. Here we show that Isl1 homologues are expressed in specific patterns in the developing zebrafish heart and play distinct roles during cardiac morphogenesis. In zebrafish, isl2a mutants show defects in cardiac looping, whereas isl2b is required for arterial pole development. Moreover, Isl2b controls the expression of key cardiac transcription factors including mef2ca, mef2cb, hand2 and tbx20. The specific roles of individual Islet family members in the development of distinct regions of the zebrafish heart renders this system particularly well-suited for dissecting Islet-dependent gene regulatory networks controlling the behavior and function of second heart field progenitors in distinct steps of cardiac development.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes