ZFIN ID: ZDB-FIG-170922-40
Liu et al., 2017 - Zebrafish B Cell Development without a Pre-B Cell Stage, Revealed by CD79 Fluorescence Reporter Transgenes. Journal of immunology (Baltimore, Md. : 1950)   199(5):1706-1715 Full text @ J. Immunol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Adult

Fig. 4

Identification of developing and mature B cells in CD79a-GFP and CD79b-GFP × Rag2-mCherry double-transgenic zebrafish. (A) B cells in young CD79a-GFP and CD79b-GFP zebrafish larvae identified by coexpression with Rag2:mCherry, analyzed by flow cytometry. Wild-type zebrafish AB line is shown as a control. Representative data of four to seven sample analyses from each day postferilization zebrafish are shown. (B) Kidney marrow of 1–8 mo CD79a-GFP × Rag2-mCherry. Dotted region is GFPmCherry. (C) Five month CD79b-GFP × Rag2-mCherry zebrafish. Low-power (top) and high-power (bottom) images of thin sections of kidney and thymus are shown. Scale bars, 200 μm (top) and 100 μm (bottom). (D) Flow cytometry analysis of adult CD79b-GFP × Rag2-mCherry zebrafish kidney and thymus. Dotted region is GFPmCherry. Frequency of CD79b:GFP+ cells in kidney is 26.4%, in thymus is 5%. Data are representative of five separate CD79b/Rag2 transgenic fish kidney analyses.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP bu3Tg; fcc89Tg standard conditions Adult head kidney IFL
mCherry bu3Tg; fcc89Tg standard conditions Adult thymus IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Immunol.