ZFIN ID: ZDB-FIG-170922-41
Liu et al., 2017 - Zebrafish B Cell Development without a Pre-B Cell Stage, Revealed by CD79 Fluorescence Reporter Transgenes. Journal of immunology (Baltimore, Md. : 1950)   199(5):1706-1715 Full text @ J. Immunol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Anatomical Terms:
Stage: Adult
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Adult

Fig. 6

Zebrafish B cell response to bacterial infection with CD79 reduction. (A) Low-power images of intestinal region of PBS control and bacteria injected CD79a-GFP fish at 1 wk postinfection. Scale bars, 1 mm. (B) Percentage of CD79a++ and CD79a+ cells in indicated tissues at 1, 2, and 4 wk postinfection; n = 3 each, mean ± SE. (C) Flow cytometry histograms of CD79a++ and CD79a+ cells in kidney and intestine tissue 1 wk postinfection, showing the clear increase in low CD79+ cells in intestine. (D) Increase in size of CD79a+ cells from intestine of infected fish, detected by flow cytometry using forward light scatter (FSC). (E) CD79a+ cells are enriched for secreted IgM compared with CD79a++ cells. RT-PCR analysis of β-actin, membrane IgH-μ (m), and secreted IgH-μ (s).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP fcc89Tg control Adult head kidney IFL
Adult intestine IFL
Adult liver IFL
Adult spleen IFL
fcc89Tg bacterial treatment by injection: Pseudomonas aeruginosa PA14 Adult head kidney IFL
Adult intestine IFL
Adult liver IFL
Adult spleen IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
fcc88Tg bacterial treatment by injection: Pseudomonas aeruginosa PA14 Adult B cell increased size, abnormal
fcc89Tg bacterial treatment by injection: Pseudomonas aeruginosa PA14 Adult intestine EGFP expression increased amount, abnormal
Adult liver EGFP expression increased amount, abnormal
Adult spleen EGFP expression increased amount, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Immunol.