ZFIN ID: ZDB-FIG-170922-39
Liu et al., 2017 - Zebrafish B Cell Development without a Pre-B Cell Stage, Revealed by CD79 Fluorescence Reporter Transgenes. Journal of immunology (Baltimore, Md. : 1950)   199(5):1706-1715 Full text @ J. Immunol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage: Adult

Fig. 3

GFP expression in CD79a and CD79b transgenic zebrafish identify B cells. (A) Diagram of production of transgenic constructs, made by long PCR from BACs containing CD79a and CD79b, where the first coding exon of each was replaced by an EGFP-PolyA segment. (B) Low power (left panels) and higher power (right panels) images of GFP expression in thin sections of kidney region of adult (4–6 mo) CD79a-GFP and CD79b-GFP zebrafish. Images were processed with Nuance software to reduce background autofluorescence. Scale bars, 100 μm (left), 200 μm (right). (C) Presence of CD79a:GFP+ and CD79b:GFP+ cells outside head kidney in adult fish. CD79a-GFP, ×4 cryosection. Scale bar, 100 μm (left), and original magnification ×10 image of cryosection. Scale bar, 200 μm (right). CD79a-GFP transgenic zebrafish also showing intestinal region. Scale bar, 200 μm. (D) Flow cytometry histogram plots of kidney marrow tissue from CD79a-GFP and CD79b-GFP lines. (E) GFP and GFP+ cell fractions were purified by electronic cell sorting from Lck-GFP, CD79a-GFP, and CD79b-GFP kidney tissue. Figure shows ethidium bromide staining of DNA amplified by RT-PCR from these samples. The lines indicate where parts of the image were joined.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP fcc88Tg standard conditions Adult head kidney IFL
Adult head kidney B cell OTHR
Adult intestine IFL
fcc89Tg standard conditions Adult head kidney IFL
Adult head kidney B cell OTHR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Immunol.