FIGURE

Fig. 2

ID

ZDB-FIG-170922-26

Publication

Walsh et al., 2017 - Progranulin regulates neurogenesis in the developing vertebrate retina

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Fig. 2

pgrn-a-targeted morpholino oligonucleotides inhibit Pgrn-a translation, which results in microphthalmia. (A) Western blot showing Pgrn-a and Actin expression in 72 hpf uninjected (UI) and morphant (5′UTR MO) embryos. (B) Histogram showing quantification of Pgrn-a expression in UI controls (26.2 ± 2.05%) and morphants (2.7 ± 0.9%); **p ≤ 0.01. Quantitative data from three biological and technical replicates is normalized to Actin and represented as mean; error bars represent the standard deviation. (C–H) Representative whole embryos from control (C–F) and experimental (G–H) groups at 72 hpf. 5′UTR MM MO and SS MM MO, embryos injected with 5-nucleotide mismatch control morpholinos; 5′UTR MO, embryos injected with pgrn-a 5′UTR-targeted morpholinos; SS MO, embryos injected with pgrn-a e3i3 splice site-targeted morpholinos.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Neurobiol.