FIGURE

Fig. S4

ID

ZDB-FIG-170829-9

Publication

Abrial et al., 2017 - TGF-β Signaling Is Necessary and Sufficient for Pharyngeal Arch Artery Angioblast Formation

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Fig. S4

A wave of TGFβ signaling is observed throughout the pharynx, Related to Figure 5.

(A-H) Spatiotemporal dynamics of TGFb signaling during PAA angioblast differentiation. Dorsal views of Tg(sox10):GFP) or Tg(sox17:GFP) embryos at the indicated developmental stages immunostained for GFP and pSMAD3 imaged in the green and far red channels to visualize neural crest (sox10) or endodermal pouches (sox17) (pseudocolored magenta) and cells experiencing active TGFβ signaling (pseudocolored yellow). 32 hpf, n=3 embryos; 36 hpf, n=6 embryos. Scale bars = 25μm.

Expression Data

Gene:	GFP
Antibody:	Ab2-smad3
Fish:	ba2Tg s870Tg
Anatomical Terms:	pharyngeal arch anatomical region pharyngeal arch 3 neural crest pharyngeal arch 4 neural crest pharyngeal arch 5 neural crest pharyngeal pouch 1 pharyngeal pouch 2 pharyngeal pouch 3 pharyngeal pouch 4 pharyngeal pouch 5 (all 9)
Stage Range:	Prim-15 to Prim-25

Expression Detail

Gene	Fish	Conditions	Stage	Anatomy	Assay
GFP	ba2Tg	standard conditions	Prim-15	pharyngeal arch 3 neural crest	IHC
GFP	ba2Tg	standard conditions	Prim-15	pharyngeal arch 4 neural crest	IHC
GFP	ba2Tg	standard conditions	Prim-15	pharyngeal arch 5 neural crest	IHC
GFP	ba2Tg	standard conditions	Prim-25	pharyngeal arch 3 neural crest	IHC
GFP	ba2Tg	standard conditions	Prim-25	pharyngeal arch 4 neural crest	IHC
GFP	ba2Tg	standard conditions	Prim-25	pharyngeal arch 5 neural crest	IHC
GFP	s870Tg	standard conditions	Prim-15	pharyngeal pouch 1	IHC
GFP	s870Tg	standard conditions	Prim-15	pharyngeal pouch 2	IHC
GFP	s870Tg	standard conditions	Prim-15	pharyngeal pouch 3	IHC
GFP	s870Tg	standard conditions	Prim-15	pharyngeal pouch 4	IHC

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Antibody Labeling

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Rep.