FIGURE

Fig. 5

ID

ZDB-FIG-170829-4

Publication

Abrial et al., 2017 - TGF-β Signaling Is Necessary and Sufficient for Pharyngeal Arch Artery Angioblast Formation

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Fig. 5

A Burst of TGF-β Signaling Precedes tie1⁺ PAA Angioblast Differentiation

(A) Cartoon of zebrafish embryo highlighting the left-side pharyngeal arches 3–6 (boxed) analyzed at higher magnifications in (B)–(N). The spatial relationships between PAA progenitors (magenta), neural-crest-derived cells (green), and endodermal pouches (gray) are shown.

(B and C) Left-side dorsal views of 32 hr post-fertilization (hpf) Tg(sox10:GFP), Tg(nkx2.5:ZsYellow), or Tg(sox17:GFP),Tg(nkx2.5:ZsYellow) embryos immunostained for GFP and ZsYellow and imaged in the green and far red channels to visualize neural crest cells (pseudocolored green), endodermal pouches (pseudocolored gray), and PAA progenitor clusters (pseudocolored magenta). n = 5 for both groups.

(D–I) Spatiotemporal dynamics of TGF-β signaling during PAA angioblast differentiation. Dorsal views of Tg(nkx2.5:GFP) embryos at the indicated developmental stages immunostained for GFP and pSMAD3 imaged in the green and far red channels to visualize PAA progenitors (pseudocolored magenta) and cells experiencing active TGF-β signaling (pseudocolored yellow). 28 hpf, n = 3; 32 hpf, n = 4; 36 hpf, n = 6.

(J) Left-side dorsal views of Tg(sox17:GFP) embryos at 36 hpf immunostained for GFP and pSMAD3 imaged in the green and far red channels to visualize endodermal pouches (pseudocolored gray) and cells active for TGF-β signaling (pseudocolored yellow), n = 2.

(K–N) Left-side dorsal views of Tg(nkx2.5:GFP) embryos at 32 hpf immunostained for GFP (pseudocolored magenta) and pSMAD3 (pseudocolored yellow) following treatment from 20 hpf with DMSO (K and L; n = 3) or 50 μM LY-364947 (M and N; n = 8). Scale bars, 25 μm.

Expression Data

Genes:	EGFP GFP ZsYellow
Antibody:	Ab2-smad3
Fish:	el83Tg s870Tg ba2Tg; fb7Tg fb7Tg; s870Tg
Condition:	chemical treatment by environment: LY 364947
Anatomical Terms:	pharyngeal arch pharyngeal arch anatomical region pharyngeal arch 3 angioblastic mesenchymal cell pharyngeal arch 3 neural crest pharyngeal arch 4 angioblastic mesenchymal cell pharyngeal arch 4 neural crest pharyngeal arch 5 angioblastic mesenchymal cell pharyngeal arch 5 neural crest pharyngeal arch 6 angioblastic mesenchymal cell pharyngeal pouch 2 pharyngeal pouch 3 pharyngeal pouch 4 pharyngeal pouch 5 (all 13)
Stage Range:	Prim-5 to Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	el83Tg
Condition:	chemical treatment by environment: LY 364947
Observed In:	pharyngeal arch anatomical region
Stage:	Prim-15

Phenotype Detail

Acknowledgments

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