FIGURE

Fig. 1

ID
ZDB-FIG-170828-12
Publication
Abrial et al., 2017 - TGF-β Signaling Is Necessary and Sufficient for Pharyngeal Arch Artery Angioblast Formation
Other Figures
All Figure Page
Back to All Figure Page
Fig. 1

A Small-Molecule Screen in Zebrafish Implicates TGF-β Signaling in PAA Morphogenesis

(A) 1,235 small molecules were screened for activities that suppress the formation of tie1+ PAA angioblasts in zebrafish. Shown are the percentages of compounds that fell into each of three categories.

(B and C) Left lateral views of tie1+ PAA angioblast clusters in 36-hr post-fertilization (hpf) embryos treated from bud stage with DMSO (B) or 10 μM CB 41227199 (C), a predicted inhibitor of TGF-β signaling. The angioblast clusters are numbered as shown (B) according to the pharyngeal arches within which they form. Seven of 14 chemically treated embryos were devoid of tie1+ clusters.

(D–L) Left lateral views of (D–G) tie1+ or (I–L) kdrl+ PAA angioblasts in 36-hpf embryos treated from 20 hpf with DMSO or the indicated, increasing concentrations of LY-364947. The angioblast clusters that did form in LY-364947-treated embryos were smaller and/or failed to sprout (asterisks in F, G, K, and L). Scale bars, 200 μm.

(H and M) Bar graphs showing the average number of tie1+ or kdrl+ angioblast clusters ±SD that formed in each experimental group (p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001). n = 40–50 embryos per group with a total of three biological replicates.

(N–Q) Left lateral views of 60-hpf Tg(kdrl:GFP),Tg(gata1:DsRed) embryos treated from 20 hpf with DMSO (N) or LY-364947 (O and P) imaged in the green and red channels to visualize endothelium (pseudocolored magenta) and red blood cells (RBC) (pseudocolored yellow), respectively. In DMSO-treated embryos, four patent PAAs supported robust blood flow as evidenced by strong luminal RBC fluorescence (N). LY-364947-treated embryos displayed a reduced number (O; class I) or complete absence (P; class II) of patent PAAs. Asterisks in (P) highlight compensatory sprouting of endothelial cells from the LDA. (Q) Bar graph showing the percentages of DMSO (n = 30) and LY-36497-treated (n = 40) embryos with four patent PAAs (wild-type), one to two patent PAAs (class I), or zero PAAs (class II). ∗∗∗p < 0.001. No animals contained three patent PAAs. Scale bars, 25 μm.

Expression Data
Genes:
Fish:
Condition:
Anatomical Terms:
Stage Range: Prim-25 to Pec-fin

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Condition:
Observed In:
Stage Range: Prim-25 to Pec-fin

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Rep.