FIGURE

Fig. 6

ID
ZDB-FIG-170815-40
Publication
Lin et al., 2017 - microRNA-206 modulates an Rtn4a/Cxcr4a/Thbs3a axis in newly forming somites to maintain and stabilize the somite boundary formation of zebrafish embryos.
Other Figures
All Figure Page
Back to All Figure Page
Fig. 6

Change of expression levels of miR-206, Rtn4al, Cxcr4a or Thbs3a fails to epithelialize somites in zebrafish embryos. (a) A diagram depicts that centrosomes of epithelial cells at the somite boundary are localized apically when epithelial cells undergo MET. (b) WT embryos at 20 hpf; (c) knockdown of miR-206, (d) overexpression of rtn4al, (e) knockdown of cxcr4a and (f) overexpression of thbs3a. Fibronectin labelled with green fluorescent signal was used to mark the somite boundary; Phalloidin labelled with red fluorescent signal was used to mark F-actin, while γ-tubulin labelled with blue fluorescent signal was used to mark centrosomes. (b–f) Three fluorescent signals were merged; (b–f′) were amplified from the corresponding panels (b–f). White arrowheads indicate centrosomes not localized apically in the epithelial cells of the defective somite boundary.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagents:
Observed In:
Stage: 20-25 somites

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Open Biol.