FIGURE

Fig. 2

ID

ZDB-FIG-170508-16

Publication

Sahu et al., 2017 - Calcium signals drive cell shape changes during zebrafish midbrain-hindbrain boundary formation

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Fig. 2

Calcium regulates cell length at the MHBC. (A–C′) Confocal images of embryos injected with mGFP and treated at 18 ss with (A, A′) DMSO, (B, B′) 100 μM 2-APB, or (C, C′) 2 μM Thaps. Embryos were washed, incubated, and live imaged at 24 ss. (A′–C′) Magnifications of A–C. Arrowheads indicate the MHBC. Asterisks in A–C indicate cell outlined in A′–C′. (D, E) Cell length quantification at the MHBC and 40 μm outside the MHBC. For statistical analysis, one-way ANOVA with Tukey’s HSD post hoc test was done. ***p < 0.001 compared with DMSO, mean ± SEM. For each measurement, DMSO (n = 29; 58 cells), 2-APB (n = 16; 32 cells), and Thaps (n = 14; 28 cells). Scale bars, 25 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT
Conditions:	chemical treatment by environment: 2-aminoethoxydiphenylborane chemical treatment by environment: thapsigargin
Observed In:	cell calcium(2+) midbrain hindbrain boundary constriction midbrain hindbrain boundary constriction cell
Stage:	20-25 somites

Phenotype Detail

Acknowledgments

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