FIGURE

Fig. S1

ID

ZDB-FIG-170505-18

Publication

Di Stefano et al., 2017 - NMN Deamidase Delays Wallerian Degeneration and Rescues Axonal Defects Caused by NMNAT2 Deficiency In Vivo

Other Figures

All Figure Page

Back to All Figure Page

Fig. S1

Protection of injured axons in NMN deamidase transgenic mice, related to Figure 3.

(A) Western blot showing the extent of NF-H degradation in distal tibial nerve of founder 14207 and 14208 (NMNd^hemi) mice 3 days after cut (relative to uncut and cut wild-type (wt) nerves).

(B) Western blot showing NF-H degradation 7 days after cut in a founder 14208-derived NMNd^hemi mouse (sterile offspring of founder 14208).

(C) Comparison of NF-H degradation in NMNd^hemi and NMNd^homo mice (founder 14209-derived line) at 2 weeks after cut. In all the blots, histone H3 was used as a loading control.

(D) Light microscopy images of sciatic nerves of an NMNd^homo mouse (founder 14209-derived line) 28 days after axotomy and

(E) a founder 14208-derived NMNd^hemi mouse 7 days after cut. Of note, protection at 7 days in the 14208-derived NMNd^hemi mouse appears stronger than that observed in line 14209-derived NMNd^hemi mice (Figure 3C). This is consistent with the higher enzymatic activity detected in tissue from founder 14208 compared to line 14209.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Curr. Biol.