FIGURE

Fig. 5

ID

ZDB-FIG-170505-16

Publication

Di Stefano et al., 2017 - NMN Deamidase Delays Wallerian Degeneration and Rescues Axonal Defects Caused by NMNAT2 Deficiency In Vivo

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Fig. 5

NMN Deamidase Protection Is Neuron Specific and Effective against Various Toxic Insults

(A) Representative bright-field images of transected neurites in wild-type, NMNd^hemi, and NMNd^homo SCG cultures. Transected neurites expressing NMN deamidase show morphological continuity several days after axotomy.

(B) Quantification of the degeneration index in experiments described in (A).

(C) Representative bright-field images of neurites in wild-type and NMNd^homo SCG cultures at the indicated time points after 20 nM vincristine addition.

(D) Quantification of the degeneration index in experiments described in (C).

(E) Representative bright-field images of neurites in wild-type and NMNd^homo SCG cultures at the indicated time points after NGF withdrawal.

(F) Quantification of the degeneration index in experiments described in (E). Degeneration index in (B), (D), and (F) was calculated from three fields per sample in three independent experiments (mean ± SD; two-way ANOVA followed by Bonferroni post hoc test, ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001, and ^∗∗∗∗p < 0.0001; NS, non-significant).