Fig. S1

Fig. S1

Protection of injured axons in NMN deamidase transgenic mice, related to Figure 3.

(A) Western blot showing the extent of NF-H degradation in distal tibial nerve of founder 14207 and 14208 (NMNd^hemi) mice 3 days after cut (relative to uncut and cut wild-type (wt) nerves).

(B) Western blot showing NF-H degradation 7 days after cut in a founder 14208-derived NMNd^hemi mouse (sterile offspring of founder 14208).

(C) Comparison of NF-H degradation in NMNd^hemi and NMNd^homo mice (founder 14209-derived line) at 2 weeks after cut. In all the blots, histone H3 was used as a loading control.

(D) Light microscopy images of sciatic nerves of an NMNd^homo mouse (founder 14209-derived line) 28 days after axotomy and

(E) a founder 14208-derived NMNd^hemi mouse 7 days after cut. Of note, protection at 7 days in the 14208-derived NMNd^hemi mouse appears stronger than that observed in line 14209-derived NMNd^hemi mice (Figure 3C). This is consistent with the higher enzymatic activity detected in tissue from founder 14208 compared to line 14209.