FIGURE

Fig. 5

ID

ZDB-FIG-170123-3

Publication

Shih et al., 2016 - Exon- and contraction-dependent functions of titin in sarcomere assembly

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Fig. 5

Generation of a ttn.2/1 near-null mutant by targeting two highly used exons. (A) Schematic representation of the ttn^xu071 mutant. Arrows indicate locations of mutations. (B) Analysis of protein extracts from 2 dpf WT larvae and ttn.2^xu065, ttn.1^xu066 and ttn^xu071 mutants. (C) qPCR with primers targeting ttn.2 and ttn.1, respectively. Means±s.d., N=5. *P<0.05, ** P<0.01. (D) The Z-disk structure marked by α-Actinin was disrupted in the ttn^xu071 mutant. (E) Electron micrographs of somites of 2 dpf WT and ttn^xu071 mutant larvae. (F) Z-disk structure was normal in ttn.2^xu065/+ and ttn.1^xu066 at 2 dpf, but sarcomere disarray was noted in ttn^xu071/+ and ttn.1^xu066. Scale bars: 20 μm (D,F) and 1 µm (E).

Expression Data

Genes:	ttn.1 ttn.2
Antibody:	Ab1-actn
Fish:	WT ttn.1^xu066/xu066 ttn.1^{xu071a/xu071a} ttn.2^xu065/xu065 ttn.2^xu065/+ ttn.1^xu071a/+; ttn.1^xu066/xu066
Anatomical Terms:	somite sarcomere somite Z disc whole organism
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ttn.1^xu066/xu066 ttn.1^{xu071a/xu071a} ttn.2^xu065/xu065 ttn.2^xu065/+ ttn.1^xu071a/+; ttn.1^xu066/xu066
Observed In:	somite sarcomere somite Z disc whole organism
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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