FIGURE

Fig. 6

ID
ZDB-FIG-170123-4
Publication
Shih et al., 2016 - Exon- and contraction-dependent functions of titin in sarcomere assembly
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Fig. 6

Phenotypes of de novo sarcomere assembly in the ttn.2/1-null mutant. (A) WT or ttnxu071 mutant embryos at the 18-somite stage were stained for α-Actinin. The images were taken at the 6th, 12th and 15th somites (ST). Scale bar: 5 μm. Z-bodies were scattered as irregular dots (open arrowheads) at the 15th somites and became periodic and aligned longitudinally (solid arrowheads) at the 12th somite. In the 6th somite, Z-bodies underwent lateral growth to form striated Z-disks (arrows) in WT embryos but not in the ttnxu071 mutant (solid arrowheads). (B) The width of the Z-disc of WT and ttnxu071 mutants at the 6th somite was quantified. Means±s.d. N=90 for each group from six embryos. **P<0.01. (C) The length of sarcomeres, marked by the distance between two adjacent Z-bodies or Z-disks, in 6th, 12th and 15th somites of WT and ttnxu071 mutant embryos. The length of sarcomeres did not increase at the 6th somite in the ttnxu071 mutant compared with WT, where sarcomere length increased to 2.2 µm. Means±s.d. ***P<0.00001. N=80 for each group in different somites from six embryos. n.s., not significant (P>0.05).

Expression Data
Antibody:
Fish:
Anatomical Terms:
Stage: 14-19 somites

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Observed In:
Stage: 14-19 somites

Phenotype Detail
Acknowledgments
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