FIGURE

Fig. 5

ID

ZDB-FIG-160425-4

Publication

Fu et al., 2016 - Imaging multicellular specimens with real-time optimized tiling light-sheet selective plane illumination microscopy

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Fig. 5

3D imaging by TLS-SPIM with real-time light sheet optimization.(a) An ideal imaging method with both high spatial resolution and imaging speed acquires spatial and temporal information simultaneously. (b) A compromised solution acquires spatial information and temporal information separately by quick switching between a high spatial resolution, low imaging speed mode and a low spatial resolution, high imaging speed mode. (c,d) 3D volume renderings of two mesodermal cells in the tailbud of a ~15 hpf zebrafish embryo imaged in a low spatial resolution, high imaging speed mode and a high spatial resolution, low imaging speed mode. (e,f) Orthogonal views of the cells in c and d. (g) Ten continuous time points of the selected area in c imaged in the low spatial resolution, high imaging speed mode, showing the lamellipodia activities of the mesodermal cell. Scale bars, 5 µm (c-f), 2 µm (g).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.